Effect of PEGylation on the biological properties of cationic carbosilane dendronized gold nanoparticles

Publication date: Available online 22 November 2019Source: International Journal of PharmaceuticsAuthor(s): Andrea Barrios-Gumiel, Javier Sánchez-Nieves, Elzbieta Pedziwiatr-Werbicka, Viktar Abashkin, Natallia Shcharbina, Dzmitry Shcharbin, Sława Glińska, Karol Ciepluch, Dorota Kuc-Ciepluch, Dominika Lach, Maria Bryszewska, Rafael Gómez, F. Javier de la MataAbstractHeterofunctionalized gold nanoparticles (AuNP) were obtained in a one pot reaction of gold precursor with cationic carbosilane dendrons (first to third generations, 1-3G) and (polyethylene)glycol (PEG) ligands in the presence of a reducing agent. The final dendron/PEG proportion on AuNPs depends on the initial dendron/PEG ratio (3/1, 1/1, 1/3) and dendron generation. AuNPs were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS), ultraviolet spectroscopy (UV-VIS), thermogravimetric analysis (TGA), nuclear magnetic resonance (1H-NMR) and zeta potential (ZP). Several assays have been carried out to determine the relevance of PEG/dendron ratio and dendron generation in the biomedical properties of PEGylated AuNPs and the results have been compared with those obtained for non-PEGylated AuNPs. Finally, analyses of PEG recognition by anti-PEG antibodies were carried out. In general, haemolysis, platelet aggregation and toxicity were reduced after PEGylation of AuNPs, the effect being dependent on dendron generation and dendron/PEG ratio. Dendron generation determines the exposure o...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research