GSE140544 Dual RNA-seq of zebrafish larvae infected with Shigella sonnei

Contributors : Vincenzo Torraca ; Myrsini Kaforou ; Jayne Watson ; Gina M Duggan ; Hazel Guerrero-Gutierrez ; Sina Krokowski ; Michael Hollinshead ; Thomas B Clarke ; Rafal J Mostowy ; Gillian S Tomlinson ; Vanessa Sancho-Shimizu ; Abigail Clements ; Serge MostowySeries Type : Expression profiling by high throughput sequencingOrganism : Danio rerio ; Shigella sonneiShigella flexneri is historically regarded as the primary agent of bacillary dysentery, yet the closely-related Shigella sonnei is replacing S. flexneri, especially in developing countries. The underlying reasons for this dramatic shift are mostly unknown. Using a zebrafish (Danio rerio) model of Shigella infection, we discover that S. sonnei is more virulent than S. flexneri in vivo. Whole animal dual-RNAseq and testing of bacterial mutants suggest that S. sonnei virulence depends on its O-antigen oligosaccharide (which is unique among Shigella species). We show in vivo using zebrafish and ex vivo using human neutrophils that S. sonnei O-antigen can mediate neutrophil tolerance. Consistent with this, we demonstrate that O-antigen enables S. sonnei to resist phagolysosome acidification and promotes neutrophil cell death. Chemical inhibition or promotion of phagolysosome maturation respectively decreases and increases neutrophil control of S. sonnei and zebrafish survival. Strikingly, larvae primed with a sublethal dose of S. sonnei are protected against a secondary lethal dose of S. sonnei in an O-antigen-dependent...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Danio rerio Shigella sonnei Source Type: research

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(University of Maryland School of Medicine) Each year, millions of people contract serious diarrheal illnesses typically from contaminated food and water. Among the biggest causes of diarrheal diseases are the bacteria Shigella and enterotoxigenic Escherichia coli (ETEC), and researchers at the University of Maryland School of Medicine are testing a vaccine designed to offer protection against these serious pathogens.
Source: EurekAlert! - Infectious and Emerging Diseases - Category: Infectious Diseases Source Type: news
Shigella spp. and entero-invasive E. coli (EIEC) use the same invasive mechanism to cause diarrheal diseases. Public health regulations apply only to Shigella spp. infections, but are hampered by the lack of simp...
Source: BMC Infectious Diseases - Category: Infectious Diseases Authors: Tags: Research article Source Type: research
Abstract For successful infection, viruses must recognize their respective host cells. A common mechanism of host recognition by viruses is to utilize a portion of the host cell as a receptor. Bacteriophage Sf6, which infects Shigella flexneri, uses lipopolysaccharide as a primary receptor and then requires interaction with a secondary receptor, a role that can be fulfilled by either outer membrane proteins (Omp) A or C. Our previous work showed that specific residues in the loops of OmpA mediate Sf6 infection. To better understand Sf6 interactions with OmpA loop variants, we determined the kinetics of these inter...
Source: Acta Virologica - Category: Infectious Diseases Authors: Tags: Acta Virol Source Type: research
(Massachusetts General Hospital) The recent discovery of an early adherence step in the infection cycle could provide a new therapeutic target or even a new method for vaccine development.
Source: EurekAlert! - Infectious and Emerging Diseases - Category: Infectious Diseases Source Type: news
This study gave insight into understanding the source water pollution accidents from the perspective of bio-hazard and biological risks, and highlighted a neglected important source of BAR in drinking water systems. PMID: 31794937 [PubMed - as supplied by publisher]
Source: Environment International - Category: Environmental Health Authors: Tags: Environ Int Source Type: research
Publication date: Available online 23 November 2019Source: Process BiochemistryAuthor(s): Yuxia Ji, Yi-Tin WangAbstractSelenium at high levels may cause adverse health effects on human beings and endanger aquatic lives due to its toxicity. Se(VI) reduction in continuous-flow reactors packed with Shigella fergusonii strain TB42616 immobilized by Ca2+-alginate gel beads was investigated under various hydraulic retention times (HRT) and influent Se(VI) concentrations. Removal efficiency up to 98.8% was achieved after 96 days operation under an HRT of 5 days and an influent Se(VI) concentration of 400 mg/L. The results ...
Source: Process Biochemistry - Category: Biochemistry Source Type: research
M. Tobin-D ’Angelo et al.
Source: Emerging Infectious Diseases Journal - Category: Infectious Diseases Source Type: research
Food-borne pathogens are a major cause of illnesses, death and expenses. Their occurrence in meat and other food is considered a global health problem. The burden of food-borne disease is increasing due to ant...
Source: BMC Research Notes - Category: Research Authors: Tags: Research note Source Type: research
In this study, we examined the genome sequences of 2239 bacteriophages from different sources for the presence of ARGs. The identified ARG-carrying bacteriophages were then analyzed by PHACTS, PHAST, and HostPhinder programs to determine their lifestyles, genes coding for bacterial cell lysis, recombinases, and a spectrum of their potential host species, respectively. We employed the SplitsTree, RDP4 and SimPlot software packages in recombination tests to identify HGT events of ARGs between these bacteriophages and bacteria. In our analyses, some ARG-carrying bacteriophages exhibited temperate and/or polyvalent patterns. T...
Source: Current Microbiology - Category: Microbiology Authors: Tags: Curr Microbiol Source Type: research
Canadian Journal of Chemistry, e-First Articles. Nanoparticles, 10 –14 nm, consisting of either Fe3O4 or Co0.2Fe2.8O4 stabilized with oleic acid, were prepared using solution combustion. Their structural and magnetic properties were examined using X-ray diffractometry, scanning electron microscopy, vibrating sample magnetometry, and Fourier-transform infrared spe ctroscopy. The properties of both sets of materials are similar, except that the cobalt-doped particles are considerably less magnetic. The in vitro inhibitory activities of the nanoparticles were assessed against pathogenic bacteria Shigella dysenteriae, Kl...
Source: Canadian Journal of Chemistry - Category: Chemistry Authors: Source Type: research
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