GSE140544 Dual RNA-seq of zebrafish larvae infected with Shigella sonnei

Contributors : Vincenzo Torraca ; Myrsini Kaforou ; Jayne Watson ; Gina M Duggan ; Hazel Guerrero-Gutierrez ; Sina Krokowski ; Michael Hollinshead ; Thomas B Clarke ; Rafal J Mostowy ; Gillian S Tomlinson ; Vanessa Sancho-Shimizu ; Abigail Clements ; Serge MostowySeries Type : Expression profiling by high throughput sequencingOrganism : Danio rerio ; Shigella sonneiShigella flexneri is historically regarded as the primary agent of bacillary dysentery, yet the closely-related Shigella sonnei is replacing S. flexneri, especially in developing countries. The underlying reasons for this dramatic shift are mostly unknown. Using a zebrafish (Danio rerio) model of Shigella infection, we discover that S. sonnei is more virulent than S. flexneri in vivo. Whole animal dual-RNAseq and testing of bacterial mutants suggest that S. sonnei virulence depends on its O-antigen oligosaccharide (which is unique among Shigella species). We show in vivo using zebrafish and ex vivo using human neutrophils that S. sonnei O-antigen can mediate neutrophil tolerance. Consistent with this, we demonstrate that O-antigen enables S. sonnei to resist phagolysosome acidification and promotes neutrophil cell death. Chemical inhibition or promotion of phagolysosome maturation respectively decreases and increases neutrophil control of S. sonnei and zebrafish survival. Strikingly, larvae primed with a sublethal dose of S. sonnei are protected against a secondary lethal dose of S. sonnei in an O-antigen-...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Danio rerio Shigella sonnei Source Type: research