Preparing monoclonal antibodies and developing immunochromatographic strips for paraquat determination in water

Publication date: Available online 16 November 2019Source: Food ChemistryAuthor(s): Yue Li, Liqiang Liu, Hua Kuang, Chuanlai XuAbstractParaquat (PQ) poisoning is a serious threat to human health that leads to pulmonary toxicity, neurotoxicity, and inflammation. Protecting humans from PQ exposure requires the development of rapid analytical methods for on-site detection. Here, two monoclonal antibodies against PQ were generated and an immunochromatographic assay (ICA) was exploited to determine PQ concentrations in water samples. The results showed that the monoclonal antibody 1D6 exhibited higher affinity and sensitivity, with an affinity constant of 5.4×108 mol/L and a limit of detection as low as 0.02 ng/mL. Without sample pretreatment, the developed ICA method provided visible limits of detection ranging from 0.25–1 ng/mL, and cut-off limits ranging from 1-5 ng/mL, where average recoveries were between 83.15% ± 1.9%-94.49% ± 2.45% with a coefficient of variation ranging from 1.40%–7.37%. Importantly, these observations were consistent with liquid chromatography tandem mass spectrometry. These data and results suggested that the ICA method was a reliable, portable, and high-throughput method for determining PQ residues in water samples.
Source: Food Chemistry - Category: Food Science Source Type: research

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Publication date: Available online 12 December 2019Source: Journal of Membrane ScienceAuthor(s): Justin Easa, Renxi Jin, Casey P. O'BrienAbstractThe evolution of carbon species on the surface and in the bulk of palladium (Pd) foils during exposure to C3H6/H2/N2 gas mixtures is quantitatively analyzed in this work as a function of temperature (300–500 °C), C3H6 concentration (5–20% in 80% H2/balance N2), and C3H6 exposure time (0–16 h) by temperature-programmed oxidation (TPO) of the carbonaceous deposits. The influence of these carbonaceous deposits on the interaction of H2 with Pd is analyzed by ...
Source: Journal of Membrane Science - Category: Materials Science Source Type: research
Publication date: Available online 12 December 2019Source: Food Science and Human WellnessAuthor(s): Yingying Zhao, Dandan Xia, Panpan Ma, Xiaofeng Gao, Wenyi Kang, Jinfeng WeiAbstractHuman health is threatened by foodborne illness and Staphylococcus aureus is a common foodborne pathogenic bacteria. It can cause food poisoning when we are infected, therefore, it is necessary to detect pathogenic bacteria. The virulence genes and detection methods of S. aureus are summarized from literatures. Traditional detection methods are simple but need bacteria enrichment and to prolong detection time. Immunological technology has hig...
Source: Food Science and Human Wellness - Category: Food Science Source Type: research
You're reading How to Become a More Positive Person, originally posted on Pick the Brain | Motivation and Self Improvement. If you're enjoying this, please visit our site for more inspirational articles. The vast majority of our behavior is habitual. As much as we feel like we are thinking throughout the day, and making conscious decisions, much of our activity is driven by deeply ingrained habits. The question is, how did these habits form? The answer lies in our subconscious mind. This is the non-thinking mind. It faithfully carries out mental commands just like a computer obediently follows digital code. The subconscio...
Source: PickTheBrain | Motivation and Self Improvement - Category: Consumer Health News Authors: Tags: featured psychology self improvement habits positive thinking Source Type: blogs
Suicide is a major public health concern in South Korea, and self-poisoning by pesticides is one of the common methods of suicide. Pesticide ban policies have been successful for suicide prevention; however, no studies have shown their effect according to ...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Home and Consumer Product Safety Source Type: news
Introduction Emergency department observation units (EDOU) have been shown to be effective in decreasing hospitalization rates and length of stay (LOS) for various conditions. However, cost savings and efficacy in the management of poisoning in EDOU have n...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Economics of Injury and Safety, PTSD, Injury Outcomes Source Type: news
Authors: Montrucchio G, Bosso S, Scanu M, Mina A, Imeneo MR, Brazzi L PMID: 31818086 [PubMed - as supplied by publisher]
Source: Minerva Anestesiologica - Category: Anesthesiology Tags: Minerva Anestesiol Source Type: research
(Duke University) Scientists from Duke University have developed a model that can predict the amount of mercury being released into a local ecosystem from deforestation. The research could point toward ways to mitigate the worst effects of mercury poisoning in regions already experiencing elevated mercury levels caused by small-scale gold mining practices, such as those in the Peruvian Amazon.
Source: EurekAlert! - Medicine and Health - Category: International Medicine & Public Health Source Type: news
Proteus mirabilis is commonly considered to be an opportunistic pathogen causing urinary tract infections (UTIs) in humans. However, some strains of P. mirabilis were found to be associated with food poisoning outbreaks, with the pathogenic mechanism still unclear. In our study, we described a novel strain of P. mirabilis C02011 isolated from patients’ specimens in a food poisoning in China. In order to determine its gastrointestinal pathogenicity, experiments were performed to compare P. mirabilis B02005 strain (isolated from healthy people) and P. mirabilis American Type Culture Collection (ATCC) 29906 strain both ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
AbstractRecently, we were consulted about a challenging case, where an infant died by poisoning and the drug-dependent mother insisted that she unintentionally gave the toxic drug through breast milk. Accordingly, we investigated the utility of immunoblotting and microRNA (miRNA) profiling of the infant ’s gastric content (GC) to differentiate between breast-feeding and formula-feeding. As a pilot study, we sampled the GC from breast-fed (GCB) and formula-fed (GCF) infants, as well as gastric juice (GJ) from fasted adults at autopsy. Breast milk (BM) samples were collected from volunteers within 1 year post-deli...
Source: International Journal of Legal Medicine - Category: Medical Law Source Type: research
Foods may potentially serve as vehicles for the transmission of antimicrobial-resistant variants of Staphylococcus aureus that are important in a human clinical context. Further, retail food products can be a cause of staphylococcal food poisoning. For these reasons and to account for source attribution and risk assessment, detailed information on the population structure, resistance, and virulence profiles of S. aureus originating from retail food products is necessary. In the current study, whole-genome sequences from 88 S. aureus isolates were subjected to bioinformatics analyses in relation to sequence types, antimicro...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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