Protective mechanism of GPR30 agonist G1 against ultraviolet B-induced injury in epidermal stem cells.

Protective mechanism of GPR30 agonist G1 against ultraviolet B-induced injury in epidermal stem cells. Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):4165-4171 Authors: Zhang Y, Li L, Xu Y, Zhao X, Li F Abstract The regeneration of the skin is vital to our wound healing and skin repair abilities. Adult epidermal stem cells (ESCs) have been shown to have the potential to renew old and dead skin cells, and ESCs have been implemented in stem cell-based therapies. GPR30 is a G protein-coupled membrane receptor for oestrogen, which has been shown to regulate cell proliferation and programmed cell death. Here, we examined the biological function of GPR30 in isolated adult murine ESCs. We show that GPR30 is fairly expressed in ESCs and is repressed upon ultraviolet B (UV-B) treatment in a dose-dependent manner. The activation of GPR30 by its agonist G1 ameliorates UV-B induced cellular oxidative stress and induction of IL-6 and IL-8. Furthermore, G1 protects against UV-B-induced cell death and improves the viability of ESCs. G1 also suppresses UV-B-induced HMGB-1 expression and protects the capacity of ESCs from the harm by UV-B radiation. Mechanistically, we show that co-treatment with G1 rescues UV-B-induced reduced Wnt1, cyclin D1 and β-catenin production, indicating the involvement of conical Wnt/β-catenin. Collectively, our data indicate that the activation of GPR30 has a protective role in ESCs, and GPR30 agonist G1-mediated ESC protection has po...
Source: Artificial Cells, Nanomedicine and Biotechnology - Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Related Links:

This article aimed to investigate effects of exosomes secreted from miRNA-29b-modified bone marrow mesenchymal stem cells (BMSCs) on SCI. Exosomes were extracted from BMSCs transfected with miRNA-29b or negative control (miR NC). SCI rats were injected intravenously with exosomes (control exosomes, miRNA-29b exosomes) and BMSCs (miR NC, miRNA-29b) through the tail vein. The expression of miRNA-29b in spinal cord tissues of SCI rats was detected by qRT-PCR. The hind limb motor function was evaluated by Basso Beattie Bresnahan (BBB) score. The histopathological damage and neuronal regeneration in spinal cord tissues was obse...
Source: Braz J Med Biol Res - Category: Research Authors: Tags: Braz J Med Biol Res Source Type: research
Publication date: Available online 11 December 2019Source: Pharmacology &TherapeuticsAuthor(s): Yinglu Guan, Guan Wang, Danielle Fails, Priyadharsini Nagarajan, Yejing GeAbstractCancer hijacks embryonic development and adult wound repair mechanisms to fuel malignancy. Cancer frequently originates from de-regulated adult stem cells or progenitors, which are otherwise essential units for postnatal tissue remodeling and repair. Cancer genomics studies have revealed convergence of multiple cancers across organ sites, including squamous cell carcinomas (SCCs), a common group of cancers arising from the head and neck, esopha...
Source: Pharmacology and Therapeutics - Category: Drugs & Pharmacology Source Type: research
We describe the generation and characterization of an in vitro progressive fibrosis model that uses cell types derived from induced pluripotent stem cells. Our model produces endogenous activated transforming growth factor β (TGF-β) and contains activated fibroblastic aggregates that progressively increase in size and stiffness with activation of known fibrotic molecular and cellular changes. We used this model as a phenotypic drug discovery platform for modulators of fibrosis. We validated this platform by identifying a compound that promotes resolution of fibrosis in in vivo and ex vivo models of...
Source: Cell Reports - Category: Cytology Source Type: research
Publication date: Available online 11 December 2019Source: Life SciencesAuthor(s): Rui Yan, JianJian Li, Ying Zhou, Li Yao, Ruya Sun, Ying Xu, Yang Ge, Guangyu AnAbstractImmunotherapy is one of the most promising strategies for cancer, compared with traditional treatments. As one of the key emerging immunotherapies, anti-PD-1/PD-L1 treatment has brought survival benefits to many advanced cancer patients. However, in pancreatic cancer, immunotherapy-based approaches have not achieved a favorable clinical effect because of mismatch repair deficiencies. Therefore, the majority of pancreatic tumors are regarded as immune-quies...
Source: Life Sciences - Category: Biology Source Type: research
This article provides a brief overview on the historical changes in our understanding of the developmental dynamics of neurogenesis and gliogenesis in the postnatal and adult mammalian brain and discusses the roles of NSCs and other progenitor populations in such cellular dynamics in health and disease of the postnatal mammalian brain.This article is categorized under: Adult Stem Cells, Tissue Renewal, and Regeneration> Stem Cell Differentiation and Reversion Adult Stem Cells, Tissue Renewal, and Regeneration> Tissue Stem Cells and Niches Adult Stem Cells, Tissue Renewal, and Regeneration> Regeneration Adult Stem ...
Source: Wiley Interdisciplinary Reviews: Developmental Biology - Category: Biology Authors: Tags: ADVANCED REVIEW Source Type: research
Publication date: 15 February 2020Source: Materials Letters, Volume 261Author(s): Pengfei Ren, Faming Wang, Tiantian Zhan, Wanjun Hu, Naizhen Zhou, Tianzhu Zhang, Jinhai YeAbstractNano-hydroxyapatite/chitosan/poly(methyl vinyl ether-alt-maleic anhydride) (NHA/CS/P(MVE-alt-MA)) composite was formed through electrostatic interaction. The results of characterization indicated that the nano-hydroxyapatite (NHA) in the composites was uniformly distributed in the polymer matrices (chitosan/poly(methyl vinyl ether-alt-maleic anhydride) (CS/P(MVE-alt-MA)). With the increase of NHA content, the porosity and strength of the composit...
Source: Materials Letters - Category: Materials Science Source Type: research
Contributors : Preethi Vijayaraj ; Aspram Minasyan ; Katherine Chung ; Weihong Yan ; David W Shia ; Thomas G Graeber ; Brigitte GompertsSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensProgressive organ fibrosis accounts for one third of all deaths worldwide, yet preclinical models that mimic the complex, progressive nature of the disease are lacking and hence there are no curative therapies. Progressive fibrosis across organs shares common cellular and molecular pathways involving chronic injury, inflammation, and aberrant repair resulting in deposition of extracellular matrix, organ ...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
In conclusion, the thermosensitive hydrogel of CS/1.0 DEX/β-GP loaded UCMSCs was a promising candidate as cell delivery vehicle for cardiac repair to reconstitute damaged myocardium.
Source: Carbohydrate Polymers - Category: Biomedical Science Source Type: research
As this article notes, researchers have recently suggested that the benefits to heart function observed over many years of stem cell therapies are not in fact due to any action of the cells themselves, not even cell signaling mechanisms such as release of exosomes, but are rather due to an immune response to the transplanted cells. The study reported here illustrates the point by showing some degree of regeneration of injured heart tissue to take place in mice when the debris of dead cells is transplanted. We might compare these findings with the body of work showing that delivery of exosomes can spur cardiac regeneration;...
Source: Fight Aging! - Category: Research Authors: Tags: Daily News Source Type: blogs
Conclusions: IL-10 deficiency/inflammation alters EPC derived exosome function, content and therapeutic effect on myocardial repair by upregulating ILK enrichment in exosomes and ILK-mediated activation of NFĸB pathway in recipient cells while ILK knockdown in exosomes attenuates NFĸB activation and reduces inflammatory response. Our study provides new understanding of how inflammation may alters stem cell-exosome mediated cardiac repair and identifies ILK as a target kinase for improving progenitor cell exosome-based cardiac therapies. PMID: 31815595 [PubMed - as supplied by publisher]
Source: Circulation Research - Category: Cardiology Authors: Tags: Circ Res Source Type: research
More News: Biotechnology | Gastroschisis Repair | Nanotechnology | Skin | Stem Cell Therapy | Stem Cells