Effect of maternal omega-3 fatty acids and vitamin E supplementation on placental apoptotic markers in rat model of early and late onset preeclampsia

Publication date: Available online 12 November 2019Source: Life SciencesAuthor(s): Vaishali Kasture, Anvita Kale, Karuna Randhir, Deepali Sundrani, Sadhana JoshiAbstractAimDisturbed placentation results in pregnancy complications like preeclampsia. Placental development is influenced by apoptosis during trophoblast differentiation and proliferation. Increased oxidative stress upregulates placental apoptosis. We have earlier reported increased oxidative stress, lower omega-3 fatty acids and vitamin E levels in women with preeclampsia. Current study examines effect of maternal omega-3 fatty acids and vitamin E supplementation on apoptotic markers across gestation in a rat model of preeclampsia.Main MethodsPregnant Wistar rats were randomly assigned to control; early onset preeclampsia (EOP); late onset preeclampsia (LOP); early onset preeclampsia + omega-3 fatty acid +vitamin E supplementation (EOP + O + E) and late onset preeclampsia + omega-3 fatty acid +vitamin E supplementation (LOP + O + E) groups. Animals (Control, EOP, EOP+O+E) were sacrificed at d14 and d20 of gestation while animals (LOP, LOP+O+E) were sacrificed at d20 to collect blood and placentae. Protein and mRNA levels of apoptotic markers were analysed by ELISA and RT-PCR respectively.Key FindingsProtein levels of proapoptotic markers like Bcl-2 associated X-protein (BAX) (p<0.05), caspase-8 and 3 (p<0.01 for both) and malondialdehyde (p<0.01) were higher only in the EOP group as compared to control....
Source: Life Sciences - Category: Biology Source Type: research