Biphasic bisperoxovanadium administration and Schwann cell transplantation for repair after cervical contusive spinal cord injury.

Biphasic bisperoxovanadium administration and Schwann cell transplantation for repair after cervical contusive spinal cord injury. Exp Neurol. 2014 Dec 12; Authors: Walker CL, Wang X, Bullis C, Liu NK, Lu Q, Fry C, Deng L, Xu XM Abstract Schwann cells (SCs) hold promise for spinal cord injury (SCI) repair; however, there are limitations for use as a lone treatment. We showed acute inhibition of the phosphatase and tensin homologue (PTEN) by bisperoxovanadium (bpV) was neuroprotective and enhanced function following cervical hemicontusion SCI. We hypothesized that combining acute bpV therapy and delayed SC engraftment would further improve neuroprotection and recovery after cervical SCI. Adult female Sprague Dawley (SD) rats were randomly sorted into 5 groups: sham, vehicle, bpV, SC transplantation, and bpV+SC transplantation. SCs were isolated from adult green fluorescent protein (GFP)-expressing SD rats (GFP-SCs). 200μg/kg bpV(pic) was administered intraperitoneally (i.p.) twice daily for 7days post-SCI in bpV-treated groups. GFP-SCs (1 x 10(6) in 5μl medium) were transplanted into the lesion epicenter at the 8th day post-SCI. Forelimb function was tested for 10weeks and histology was assessed. bpV alone significantly reduced lesion (by 40%, p<0.05) and cavitation (by 65%, p<0.05) and improved functional recovery (p <0.05) compared to injury alone. The combination promoted similar neuroprotection (p<0.01 vs. injury); h...
Source: Experimental Neurology - Category: Neurology Authors: Tags: Exp Neurol Source Type: research