Quantitative determination of human IgA subclasses and their Fc-glycosylation patterns in plasma by using peptide analogue internal standard and ultra-high-performance liquid chromatography-triple quadrupole mass spectrometry.

CONCLUSIONS: The developed method showed good validation results, and the absolute quantification results of IgA correlated to those from ELISA. Th pilot application study showed that IgA and IgA glycopeptides can be potential biomarker candidates for kidney diseases, and more clinical samples application are worth of investigation.
PMID: 31705576 [PubMed - as supplied by publisher]

Source: Rapid Communications in Mass Spectrometry : RCM - November 7, 2019 Category: Chemistry Authors: Chen HF, Shiao CY, Wu MY, Lin YC, Chen HH, Chang WC, Wu MS, Kao CC, Tsai IL Tags: Rapid Commun Mass Spectrom Source Type: research