Generation of two ERF gene knockout human embryonic stem cell lines using CRISPR/Cas9 System

Publication date: Available online 6 November 2019Source: Stem Cell ResearchAuthor(s): Lihui Si, Ruiqi Yang, Jian Liu, Yubo Dong, Hongtao Zhang, Xiaohong XuAbstractHuman ERF gene is a transcription factor involved in development, trophoblast differentiation, apoptosis, and cancer progress. To further understand the exact roles of ERF in these processes, here we report that establishment of two ERF knockout human embryonic stem cell (hESC) lines by CRISPR/Cas9 mediated gene targeting. These cell lines exhibited classical hESC morphology and normal karyotype, and highly expressed pluripotent markers, and had differentiation potential in vitro. These cell lines provide good materials to understand the roles of ERF in development, trophoblast differentiation and craniosynostosis for further studies.
Source: Stem Cell Research - Category: Stem Cells Source Type: research