PulseNet and the Changing Paradigm of Laboratory-Based Surveillance for Foodborne Diseases.

This article highlights the contributions made by public health laboratories in transforming PulseNet's surveillance and describes how the transformation is changing local and national surveillance practices. Our data show that WGS is better at identifying clusters than PFGE, especially for clonal organisms such as Salmonella Enteritidis. The need to develop prioritization schemes for cluster follow-up and additional resources for both public health laboratory and epidemiology departments will be critical as PulseNet implements WGS for foodborne disease surveillance in the United States. PMID: 31682558 [PubMed - in process]
Source: Public Health Reports - Category: International Medicine & Public Health Tags: Public Health Rep Source Type: research

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Contamination of edible produce leaves with human bacterial pathogens has been associated with serious disease outbreaks and has become a major public health concern affecting all aspects of the market, from farmers to consumers. While pathogen populations residing on the surface of ready-to-eat produce can be potentially removed through thorough washing, there is no disinfection technology available that effectively eliminates internal bacterial populations. By screening 303 multi-gene deletion (MGD) mutants of Salmonella enterica serovar Typhimurium (STm) 14028s, we were able to identify ten genomic regions that play a r...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In this study, 43 Salmonella phages were isolated from environmental resources and among them, phage D1-2 was selected since it exhibited the most potent lytic ability and the broadest host spectrum against tested Salmonella strains. Further study demonstrated that D1-2 shows high pH and thermal tolerances and a short latent period, together with a low frequency of emergence of phage resistance. D1-2 effectively inhibited the growth of two MDR Salmonella strains in liquid egg white and egg yolk at both 4 °C and 25 °C. Morphology and phylogeny indicated that D1-2 belongs to the Myoviridae family. Genome analysis of ...
Source: Food Research International - Category: Food Science Source Type: research
The objective of this study was to study the inactivation kinetics of Escherichia coli O157:H7 and Salmonella Typhimurium under microwave pasteurization at temperatures between 80 and 90 °C, i.e., at conditions that are employed in conventional pasteurization. Inoculated juices were treated at different power levels (600 W, 720 W) and treatment times (5s, 10s, 15s, 20s, 25s). Time-temperature profiles were obtained by fiber-optic sensors in contact with the samples allowing continuous data collection. The log-logistic and Arrhenius equations were used to account for the influence of the temperature histo...
Source: Food Microbiology - Category: Food Science Authors: Tags: Food Microbiol Source Type: research
Publication date: Available online 17 January 2020Source: Microchemical JournalAuthor(s): Zahra Rahimi Nadi, Taghi Zahraei Salehi, Iradj Ashrafi Tamai, Abbas Rahimi foroushani, Mika Sillanpaa, Mohammad Mehdi Soltan DallalAbstractSalmonella is one of the major causes of foodborne outbreaks and numerous studies have reported the presence of its serovars in foodborne outbreaks. Identification of the most prevalent serovars, common polluted food types and attention to the antibiotic resistance of its serovars, are therefore key factors in the prevention and control of salmonellosis. The aim of this study is thus isolation of t...
Source: Microchemical Journal - Category: Chemistry Source Type: research
Publication date: Available online 15 January 2020Source: Molecular and Cellular ProbesAuthor(s): Kasanchi M. Momin, Arockiasamy Arun Prince Milton, Sandeep Ghatak, Shiny C. Thomas, Govindarajan Bhuvana Priya, Samir Das, Ingudam Shakuntala, Rajkumari Sanjukta, Kekungu-u Puro, Arnab SenAbstractThe polymerase spiral reaction (PSR), a novel isothermal method for targeted DNA amplification, was effectively applied to detect Salmonella in artificially spiked pork. The specificity of the developed PSR was tested using 16 Salmonella and 15 non-Salmonella strains. The PSR assay was 10-fold more sensitive than conventional end-poin...
Source: Molecular and Cellular Probes - Category: Molecular Biology Source Type: research
This article is protected by copyright. All rights reserved. PMID: 31944569 [PubMed - as supplied by publisher]
Source: Biotechnology Journal - Category: Biotechnology Authors: Tags: Biotechnol J Source Type: research
In this study, 8 Salmonella phages and 10 E. coli O157:H7 phages were isolated from chicken products. A polyvalent phage PS5 capable of infecting S. Enteritidis, S. Typhimurium, and E. coli O157:H7 was further characterized and its efficacy in reducing these foodborne pathogens was evaluated in in vitro and in foods. Morphology, one-step growth, and stability assay showed that phage PS5 was a myovirus, with relatively short latent periods, large burst sizes, and high stability. Genome sequencing analysis revealed that the genome of PS5 does not contain any genes associated to antibiotic resistance, toxins, lysogeny, and vi...
Source: Food Research International - Category: Food Science Source Type: research
Food contamination with Salmonella enterica and enterohemorrhagic Escherichia coli is among the leading causes of foodborne illnesses worldwide and crop plants are associated with> 50% of the disease outbreaks. ...
Source: BMC Plant Biology - Category: Biology Authors: Tags: Research article Source Type: research
In this study, we set out to compare which of the currently available open-source command-line tools is most suitable to replace serological testing. A thorough evaluation of the differing computational approaches is highly important to ensure the backward compatibility of serotyping data and to maintain the comparability between laboratories. PMID: 31862714 [PubMed - as supplied by publisher]
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research
Canadians cooking with poultry are being warned by the Public Health Agency of Canada to take extra care as a nation-wide salmonella outbreak continues.
Source: CBC | Health - Category: Consumer Health News Tags: News/Canada/PEI Source Type: news
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