Conformational properties of L-fucose and the tetrasaccharide building block of the sulfated L-fucan from Lytechinus variegatus

Publication date: Available online 4 November 2019Source: Journal of Structural BiologyAuthor(s): Francisco F. Bezerra, William P. Vignovich, AyoOluwa O. Aderibigbe, Hao Liu, Joshua S. Sharp, Robert J. Doerksen, Vitor H. PominAbstractAlthough the 3D structure of carbohydrates is known to contribute to their biological roles, conformational studies of sugars are challenging because their chains are flexible in solution and consequently the number of 3D structural restraints is limited. Here, we investigate the conformational properties of the tetrasaccharide building block of the Lytechinus variegatus sulfated fucan composed of the following structure [L-Fucp4(SO3−)-α(1-3)-L-Fucp2,4(SO3−)-α(1-3)-L-Fucp2(SO3−)-α(1-3)-L-Fucp2(SO3−)] and the composing monosaccharide unit Fucp, primarily by NMR experiments performed at very low temperatures and using H2O as the solvent for the sugars rather than using the conventional deuterium oxide. By slowing down the fast chemical exchange rates and forcing the protonation of labile sites, we increased the number of through-space 1H–1H distances that could be measured by NMR spectroscopy. Following this strategy, additional conformational details of the tetrasaccharide and L-Fucp in solution were obtained. Computational molecular dynamics was performed to complement and validate the NMR-based measurements. A model of the NMR-restrained 3D structure is offered for the tetrasaccharide.Graphical abstract
Source: Journal of Structural Biology - Category: Biology Source Type: research