Cancers, Vol. 11, Pages 1691: Automated Workflow for Somatic and Germline Next Generation Sequencing Analysis in Routine Clinical Cancer Diagnostics
Cancers, Vol. 11, Pages 1691: Automated Workflow for Somatic and Germline Next Generation Sequencing Analysis in Routine Clinical Cancer Diagnostics
Cancers doi: 10.3390/cancers11111691
Authors:
Muscarella
Fabrizio
De Bonis
Mancini
Balsamo
Graziano
Centra
Sparaneo
Trombetta
Bonfitto
Scagliusi
Larizza
Capoluongo
Fazio
Thanks to personalized medicine trends and collaborations between industry, clinical research groups and regulatory agencies, next generation sequencing (NGS) is turning into a common practice faster than one could have originally expected. When considering clinical applications of NGS in oncology, a rapid workflow for DNA extraction from formalin-fixed paraffin-embedded (FFPE) tissue samples, as well as producing high quality library preparation, can be real challenges. Here we consider these targets and how applying effective automation technology to NGS workflows may help improve yield, timing and quality-control. We firstly evaluated DNA recovery from archived FFPE blocks from three different manual extraction methods and two automated extraction workstations. The workflow was then implemented to somatic (lung/colon panel) and germline (BRCA1/2) library preparation for NGS analysis exploiting two automated workstations. All commercial kits gave good results in terms of DNA yield and quality. On the other hand, the automated workstation workflow has been proven to be a valid automatic extraction system to obtain ...
Source: Cancers - Category: Cancer & Oncology Authors: Muscarella Fabrizio De Bonis Mancini Balsamo Graziano Centra Sparaneo Trombetta Bonfitto Scagliusi Larizza Capoluongo Fazio Tags: Article Source Type: research