Synthesis of titanium oxide nanoparticles using Aloe barbadensis mill and evaluation of its antibiofilm potential against Pseudomonas aeruginosa PAO1

Publication date: Available online 28 October 2019Source: Journal of Photochemistry and Photobiology B: BiologyAuthor(s): J. Rajkumari, C. Maria Magdalane, B. Siddhardha, J. Madhavan, G. Ramalingam, Naif Abdullah Al-Dhabi, Mariadhas Valan Arasu, A.K.M. Ghilan, V. Duraipandiayan, K. KaviyarasuAbstractTitanium dioxide nanoparticles (TiO2-NPs) were synthesized using the aqueous leaf extract of Aloe barbadensis as a reducing and fabricating agent. The biosynthesis of the TiO2-NPs was initially confirmed by UV–vis spectroscopy. Based on the HRTEM and FESEM analysis, the biosynthesized NPs were found to be polydispersed and predominantly spherical in shape, with an average size of ~20 nm. A sharp and strong characteristic peaks of titanium (Ti) and oxygen (O) observed in the EDS pattern confirmed the synthesis of the TiO2-NPs. The FTIR spectroscopy suggested the presence of terpenoids, flavonoids and proteins which might be responsible for the biosynthesis and fabrication of the TiO2-NPs. The crystalline nature of the synthesized TiO2-NPs constituting of a mixture of brookite, anatase, and rutile phases was indicated by the XRD pattern. The spectral window around 180–1000 cm−1 covered the high-frequency Raman spectra of the TiO2-NPs. The Raman vibrational spectrum showed four Eg modes centered at 197.84, 399.24, 514.50, and 641.58 cm−1 representing the anatase phase of TiO2-NPs. The strongest and broadened peak of anatase was observed at the frequency of 641.58 cmâˆ...
Source: Journal of Photochemistry and Photobiology B: Biology - Category: Speech-Language Pathology Source Type: research