Biosynthetic machinery of ionophore polyether lasalocid: enzymatic construction of polyether skeleton.

Biosynthetic machinery of ionophore polyether lasalocid: enzymatic construction of polyether skeleton.
Curr Opin Chem Biol. 2013 Jun 21;
Authors: Minami A, Oguri H, Watanabe K, Oikawa H
Abstract
Diversity of natural polycyclic polyethers originated from very simple yet versatile strategy consisting of epoxidation of linear polyene followed by epoxide opening cascade. To understand two-step enzymatic transformations at molecular basis, a flavin containing monooxygenase (EPX) Lsd18 and an epoxide hydrolase (EH) Lsd19 were selected as model enzymes for extensive investigation on substrate specificity, catalytic mechanism, cofactor requirement and crystal structure. This pioneering study on prototypical lasalocid EPX and EH provides insight into detailed mechanism of ionophore polyether assembly machinery and clarified remaining issues for polyether biosynthesis.
PMID: 23796908 [PubMed - as supplied by publisher]

Source: Current Opinion in Chemical Biology - June 21, 2013 Category: Biochemistry Authors: Minami A, Oguri H, Watanabe K, Oikawa H Tags: Curr Opin Chem Biol Source Type: research