Molecules, Vol. 24, Pages 3793: Surface Display of Antigen Protein VP8* of Porcine Rotavirus on Bacillus Subtilis Spores Using CotB as a Fusion Partner

In this study, a shuttle vector was constructed for the spore surface display of the spike protein VP8* from porcine rotavirus (the genotype was G5P[7]). A successful display of the CotB-VP8* fusion protein on the spore surface was confirmed by Western blot and immunofluorescence microscopy analysis. The capacity for immune response generated after immunization with the recombinant strain was evaluated in a mouse model. The intestinal fecal IgA and serum IgG were detected by enzyme-linked-immunosorbent serologic assay (ELISA). Importantly, recombinant strain spores could elicit strong specific mucosal and humoral immune responses. These encouraging results suggest that recombinant B. subtilis BV could provide a strategy for a potential novel application approach to the development of a new and safe mucosal subunit vaccine against porcine rotavirus.

Source: Molecules - October 21, 2019 Category: Chemistry Authors: Wanqiang Li Jie Feng Jiajun Li Jianzhen Li Zhenhua Wang Abdul Khalique Miao Yang Xueqin Ni Dong Zeng Dongmei Zhang Bo Jing Qihui Luo Kangcheng Pan Tags: Article Source Type: research