Synthesis and Characterization of Silver Nanoparticles-doped Hydroxyapatite/Alginate Microparticles with Promising Cytocompatibility and Antibacterial Properties

Publication date: Available online 11 October 2019Source: Colloids and Surfaces A: Physicochemical and Engineering AspectsAuthor(s): Qiuju Zhou, Tianwen Wang, Can Wang, Zheng Wang, Yanan Yang, Pei Li, Ruihua Cai, Meng Sun, Hongyu Yuan, Lei NieAbstractIn this paper, a facile double-emulsion approach was reported to fabricate the silver nanoparticles (AgNPs)-doped hydroxyapatite/alginate nanocomposite microparticles. First, hydroxyapatite nanoparticles (HApNPs) were prepared using coprecipitation method, then, HApNPs were involved in sodium alginate (Alg) network to form HAp/Alg microparticles via emulsion process. Next, silver nitrate was involved into HAp/Alg microparticles using second emulsion, which ascorbic acid was used as a trigger to form in situ-AgNPs-doped HAp/Alg microparticles. Physicochemical properties of the nanocomposite microparticles were characterized by Transmission Electron Microscopy (TEM), Fourier Transform Infrared (FT-IR) spectra, Dynamic Light Scattering (DLS), Ultraviolet-Visible (UV-Vis) Absorption Spectra, and Thermogravimetric analysis (TG). It was demonstrated that AgNPs and HApNPs were evenly distributed in entire microparticles (diameter ∼550 nm). Furthermore, the AgNPs-doped HAp/Alg microparticles showed a good cytocompatibility by culturing with A549 cells. Finally, the microparticles displayed an excellent antibacterial activity against Gram-negative E. coli and Gram-positive S. aureus. Above results manifested the significance of the fi...
Source: Colloids and Surfaces A: Physicochemical and Engineering Aspects - Category: Chemistry Source Type: research