Viruses, Vol. 11, Pages 935: Quantitative Microscopy Reveals Stepwise Alteration of Chromatin Structure during Herpesvirus Infection

Viruses, Vol. 11, Pages 935: Quantitative Microscopy Reveals Stepwise Alteration of Chromatin Structure during Herpesvirus Infection
Viruses doi: 10.3390/v11100935
Authors:
Aho
Mäntylä
Ekman
Hakanen
Mattola
Chen
Weinhardt
Ruokolainen
Sodeik
Larabell
Vihinen-Ranta

During lytic herpes simplex virus 1 (HSV-1) infection, the expansion of the viral replication compartments leads to an enrichment of the host chromatin in the peripheral nucleoplasm. We have shown previously that HSV-1 infection induces the formation of channels through the compacted peripheral chromatin. Here, we used three-dimensional confocal and expansion microscopy, soft X-ray tomography, electron microscopy, and random walk simulations to analyze the kinetics of host chromatin redistribution and capsid localization relative to their egress site at the nuclear envelope. Our data demonstrated a gradual increase in chromatin marginalization, and the kinetics of chromatin smoothening around the viral replication compartments correlated with their expansion. We also observed a gradual transfer of capsids to the nuclear envelope. Later in the infection, random walk modeling indicated a gradually faster transport of capsids to the nuclear envelope that correlated with an increase in the interchromatin channels in the nuclear periphery. Our study reveals a stepwise and time-dependent mechanism of herpesvirus nuclear egress, in which progeny viral capsids approach the egress site...

Source: Viruses - October 10, 2019 Category: Virology Authors: Aho M äntylä Ekman Hakanen Mattola Chen Weinhardt Ruokolainen Sodeik Larabell Vihinen-Ranta Tags: Article Source Type: research