Molecular detection of Anaplasma phagocytophilum in wild carnivores in north-eastern Poland

Anaplasma phagocytophilum is an obligate parasitic intracellular bacterium. It is the causative agent of granulocytic anaplasmosis, with effects on human and animal health. In Europe, the pathogen is mainly trans...
Source: Parasites and Vectors - Category: Microbiology Authors: Tags: Short report Source Type: research

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In this study, the full length of DNA encoding A. marginale MSP4 (AmMSP4) was cloned from the parasites. The open reading frame of msp4 coding sequence of Thailand strain is 849 bp. Phylogenetic analysis revealed that the msp4 coding sequence of A. marginale was highly conserved when compared with Anaplasma phagocytophilum. The recombinant plasmid was further transformed into the BL21-CodonPlus (DE3)-RIPL competent cells for over-expression of the recombinant major surface protein 4 of A. marginale (rAmMSP4). Sera from rabbit immunized with rAmMSP4 and from cattle infected with A. marginale were used to study the antigenic...
Source: Acta Tropica - Category: Infectious Diseases Authors: Tags: Acta Trop Source Type: research
In this study, we applied complementary in silico approaches to modeling how Anaplasma phagocytophilum infection modulates tick vector regulome. This proof-of-concept research provided support for the use of network analysis in the study of regulome response to infection, resulting in new information on tick-pathogen interactions and potential targets for developing interventions for the control of tick infestations and pathogen transmission. Deciphering the precise nature of circuits that shape the tick regulome in response to pathogen infection is an area of research that in the future will advance our knowledge of tick-...
Source: Frontiers in Physiology - Category: Physiology Source Type: research
In this study Anaplasmosis (14%) was highly prevalent during the winter season and Babesiosis (13.73%) was highly prevalent during summer months followed by Anaplasmosis (9.8%) and Theileriosis (7.8%), the lowest prevalence of Trypanosomiasis was observed during the rainy season. The s easonal variation in prevalence of haemoprotozoan disease might be due to influence of climatic factors on density of vector population in that geographical area. Haemogram revealed decreased level of haemoglobulin, packed cell volume and total erythrocyte count. The serum biochemistry revealed elev ated level of liver enzyme Asparate transa...
Source: Journal of Parasitic Diseases - Category: Parasitology Source Type: research
This study provides basic knowledge that is necessary to initiate Kyasanur Forest Disease (KFD) prevention programs in these areas. Ticks were sampled from Wayanad districts of Kerala from domestic animals and identified morphologically. A total of 195 cattle searched, in which 168 (86.15%) cattle were infested with ticks and a total of 3633 ticks comprising three genera and seven species were collected,Rhipicephalus microplus (52.71%) was prevalent species followed byHaemaphysalis bispinosa (16.9%),Rhipicephalus decoloratus (15.77%),Haemaphysalis turturis (11.42%),Rhipicephalus sanguineus (1.32%),Amblyomma integrum (1.15%...
Source: Journal of Parasitic Diseases - Category: Parasitology Source Type: research
This study aimed at investigating the occurrence of Anaplasma spp. in domestic cats from Greater Rio de Janeiro, and evaluating hematological changes associated with this rickettsial infection. Were sampled 216 cats, we performed nested PCR (nPCR) and real-time PCR (qPCR) assays targeting A. platys-16S-rDNA, A. platys-gltA and A. phagocytophilum-msp2 sequences. As evaluated with gltA-qPCR the frequency of cats positive for A. platys was 3.7% (n = 8/216) and by 16S-rDNA nested-PCR it was 0.9% (n = 2/216). No cats were positive to msp2-qPCR to A. phagocytophilum. The sequences of A. platys present...
Source: Acta Tropica - Category: Infectious Diseases Authors: Tags: Acta Trop Source Type: research
Anaplasma ovis is a major cause of small ruminant anaplasmosis, a tick-borne disease mainly affecting small ruminants in tropical and subtropical regions of the world. Due to health and production problems in dai...
Source: Parasites and Vectors - Category: Microbiology Authors: Tags: Research Source Type: research
Publication date: December 2018Source: Parasitology International, Volume 67, Issue 6Author(s): Shola David Ola-Fadunsin, Fufa Ido Gimba, Donea Abdurazak Abdullah, Reuben Sunil Kumar Sharma, Faez Jesse Firdaus Abdullah, Rehana Abdullah SaniAbstractBovine anaplasmosis is a major concern to cattle farming in most parts of the world. Anaplasmosis negatively impacts the profitability of cattle farming by reducing the production, reproduction, and draft ability of cattle. Here, we report results from a one-year cross sectional study to determine the epidemiology and the risk factors for Anaplasma marginale infection of cattle i...
Source: Parasitology International - Category: Parasitology Source Type: research
This study sought to detect and characterize hemoparasites among farms in acaricide-failure hotspots of central as compared to the acaricide-failure naïve areas in eastern Uganda. Nested PCR assays were performed to determine the prevalences of Babesia bovis, Babesia bigemina, Theileria parva, and Anaplasma marginale in cattle blood samples sourced from randomly selected farms. Randomly selected isolates were sequenced to determine the genetic diversity of the parasites using the following marker genes: B. bovis spherical body protein 4, B. bigemina rhoptry-associated protein 1a, T. parva 104 kDa microneme-rhoptry a...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
Publication date: December 2018Source: Parasitology International, Volume 67, Issue 6Author(s): Shola David Ola-Fadunsin, Fufa Ido Gimba, Donea Abdurazak Abdullah, Reuben Sunil Kumar Sharma, Faez Jesse Firdaus Abdullah, Rehana Abdullah SaniAbstractBovine anaplasmosis is a major concern to cattle farming in most parts of the world. Anaplasmosis negatively impacts the profitability of cattle farming by reducing the production, reproduction, and draft ability of cattle. Here, we report results from a one-year cross sectional study to determine the epidemiology and the risk factors for Anaplasma marginale infection of cattle i...
Source: Parasitology International - Category: Parasitology Source Type: research
This study sought to detect and characterize hemoparasites among farms in acaricide-failure hotspots of central as compared to the acaricide-failure naïve areas in eastern Uganda. Nested PCR assays were performed to determine the prevalences of Babesia bovis, Babesia bigemina, Theileria parva, and Anaplasma marginale in cattle blood samples sourced from randomly selected farms. Randomly selected isolates were sequenced to determine the genetic diversity of the parasites using the following marker genes: B. bovis spherical body protein 4, B. bigemina rhoptry-associated protein 1a, T. parva 104 kDa microneme-rhoptry a...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
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