Develop and Field Evolution of Single Tube Nested PCR, SYBRGreen PCR Methods, for the Diagnosis of Leprosy in Paraffin-embedded Formalin Fixed Tissues in Yunnan Province, a Hyper endemic Area of Leprosy in China

ConclusionsThese findings suggest that STNPCR or SYBRGreen PCR (131-bp and 101-bp fragment amplification, respectively) for RLEP using FFPE specimens performs better as a diagnostic test and for monitoring response to MDT than does simple PCR based on 372-bp fragment amplification. Additionally, STNPCR showed increased sensitivity for PB diagnosis using FFPE specimens, which can be transferred remotely or retrieved from previous leprosy patients.
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research

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Publication date: July 2019Source: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, Volume 128, Issue 1Author(s): Dr. Neetha Santosh, Dr. Amber Kiyani, Dr. Uzair Luqman, Dr. Gulraiz Zulfiqar, Dr. Umar Farooq, Dr. Nadia ZaibProtozoal, invasive fungal and mycobacterial infections in the head and neck region are exceedingly rare in the developed world. However, in developing countries, endemic infections may involve the oral mucosa or facial skin. Here we present three unusual infections involving these sites. Case 1: A 48-year-old male presented with difficulty in swallowing for a couple of months. Intraoral e...
Source: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology - Category: ENT & OMF Source Type: research
LITFL • Life in the Fast Lane Medical Blog LITFL • Life in the Fast Lane Medical Blog - Emergency medicine and critical care medical education blog aka Tropical Travel Trouble 008 Peer Reviewer Dr McBride ID physician, Wisconsin TB affects 1/3rd of the population and one patient dies every 20 seconds from TB. Without treatment 50% of pulmonary TB patients will be dead in 5 years. In low to middle income countries both TB and HIV can be ubiquitous, poor compliance can lead to drug resistance and malnourished infants are highly susceptible. TB can be very complex and this post will hopefully give you the backbone t...
Source: Life in the Fast Lane - Category: Emergency Medicine Authors: Tags: Clinical Cases Tropical Medicine Genexpert meningitis TB TB meningitis Tuberculosis Source Type: blogs
ConclusionThe prevalence of HL was similar to previous reports. However, all histoid patients were “de novo” cases, differing from published studies. Fusocellular macrophage transformation could be explained by the differences in cytoskeleton proteins expressed in histoid lesions in comparison to other leprosy variants, with emphasis on vimentin and smooth muscle actin.
Source: International Journal of Dermatology - Category: Dermatology Authors: Tags: Tropical Medicine Rounds Source Type: research
Authors: Marques LÉC, Frota CC, Quetz JDS, Bindá AH, Mota RMS, Pontes MAA, Gonçalves HS, Kendall C, Kerr LRFS Abstract Mycobacterium leprae bacilli are mainly transmitted by the dissemination of nasal aerosols from multibacillary (MB) patients to susceptible individuals through inhalation. The upper respiratory tract represents the main entry and exit routes of M. leprae. Therefore, this study aimed to evaluate the sensitivity and specificity of real-time quantitative polymerase chain reaction (qPCR) in detecting M. leprae in nasal secretion (NS) and skin biopsy (SB) samples from MB and pauciba...
Source: Pathogens and Global Health - Category: International Medicine & Public Health Tags: Pathog Glob Health Source Type: research
by Amanda N. B. Fontes, Luana N. G. C. Lima, Rosa M. S. Mota, Rosa L. F. Almeida, Maria A. Pontes, Heitor de S. Gon çalves, Cristiane C. Frota, Varalakshmi D. Vissa, Patrick J. Brennan, Ricardo J. P. S. Guimaraes, Carl Kendall, Ligia R. F. S. Kerr, Philip N. Suffys Leprosy is endemic in large part of Brazil with 28,761 new patients in 2015, the second largest number worldwide and reaches 9/10.000 in highly endemic regions and 2.7/10.000 in the city of Fortaleza, Ceará, Northeast Brazil. For better understanding of risk factors for leprosy transmission, we c onducted an epidemiologic study supplemented by 17 ...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
CONCLUSION: This case highlights that the lepromatous iridocyclitis, a serious vision-threatening disorder requires a high index of suspicion for prompt diagnosis in atypical cases particularly in endemic areas. Examination of the aqueous humor can also be helpful in diagnosis. PMID: 29090057 [PubMed]
Source: Journal of Ophthalmic and Vision Research - Category: Opthalmology Tags: J Ophthalmic Vis Res Source Type: research
A 33-year-old Indonesian man who was visiting Japan as a trainee in agricultural sector presented to the outpatient department of dermatology with a 1-year history of progressive rash. The rash had originated on his trunk and had gradually spread to his chest, abdomen, back and arms (Figure 1). The patient was not on any medications. He also denied any history of an allergy. Polymorphic, confluent, partially raised, hypopigmented macules that had progressed slowly were observed. The lesions demonstrated associated scaling, alopecia, and anesthesia. Although a left greater auricular nerve enlargement was detected, the patie...
Source: QJM - Category: Internal Medicine Source Type: research
Conclusions/SignificanceThis is the first report of usingM.leprae whole genome sequencing to reveal that treated and cured leprosy patients who remain in endemic areas can be reinfected by another strain. Next generation sequencing can be applied reliably toM.leprae DNA extracted from biopsies to discriminate between cases of relapse and reinfection, thereby providing a powerful tool for evaluating different outcomes of therapeutic regimens and for following disease transmission.
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
Abstract A 60-year-old Nigerian man, who had lived in Europe for 30 years but had returned home frequently, presented with right frontalis muscle weakness and right ulnar nerve palsy, without skin lesions. Neurophysiology showed a generalised neuropathy with demyelinating features. Blood tests were positive for HIV, with a normal CD4 count. There was nerve thickening both clinically and on MRI. Nerve biopsy showed chronic endoneuritis and perineuritis (indicating leprosy) without visible mycobacteria. His neuropathy continued to deteriorate (lepra reaction) before starting treatment with WHO multidrug therapy, hig...
Source: Practical Neurology - Category: Neurology Authors: Tags: Pract Neurol Source Type: research
by Camilo Beltr án-Alzate, Fernando López Díaz, Marcela Romero-Montoya, Rama Sakamuri, Wei Li, Miyako Kimura, Patrick Brennan, Nora Cardona-Castro An active search forMycobacterium leprae drug resistance was carried out, 243 multibacillary patients from endemic regions of Colombia were included from 2004 to 2013 in a surveillance program. This program was a World Health Organization initiative for drug resistance surveillance in leprosy, where Colombia is a sentinel country.M. leprae DNA from slit skin smear and/or skin biopsy samples was amplified and sequenced to identify mutations in the drug resis...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
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