Construction of chromosome segment substitution lines of Dongxiang common wild rice (Oryza rufipogon Griff.) in the background of the japonica rice cultivar Nipponbare (Oryza sativa L.)

In this study, we used Dongxiang common wild rice as the donor parent and Nipponbare (Oryza sativa L), a reference-sequence japonica cultivar, as the recurrent parent to develop a set of 104 chromosome segment substitution lines (CSSLs) through crossing and backcrossing with marker-assisted selection based on 203 polymorphic molecular markers evenly distributed across 12 chromosomes. The 104 CSSLs covered 87.94% of the genome of Dongxiang common wild rice. Each CSSL contained an average of four introgressed segments, and the average segment length was 3.3 Mb. Quantitative trait locus (QTL) analysis of the panicle-related traits of the CSSLs at two sites (Beijing and Nanchang, Jiangxi Province) revealed 18 QTLs for eight traits. Among these 18 QTLs were two known grain length- and width-related genes and four novel QTLs. In addition, two QTLs were verified, and two novel QTLs were identified, for panicle neck length, a domestication-related trait. The developed CSSLs potentially represent a valuable population for detecting favorable genes in common wild rice and creating germplasm resources for japonica rice breeding.
Source: Plant Physiology and Biochemistry - Category: Biochemistry Source Type: research