Innate and adaptive nasal mucosal immune responses following experimental human pneumococcal colonization

Streptococcus pneumoniae (Spn) is a common cause of respiratory infection, but also frequently colonizes the nasopharynx in the absence of disease. We used mass cytometry to study immune cells from nasal biopsy samples collected following experimental human pneumococcal challenge in order to identify immunological mechanisms of control of Spn colonization. Using 37 markers, we characterized 293 nasal immune cell clusters, of which 7 were associated with Spn colonization. B cell and CD161+CD8+ T cell clusters were significantly lower in colonized than in noncolonized subjects. By following a second cohort before and after pneumococcal challenge we observed that B cells were depleted from the nasal mucosa upon Spn colonization. This associated with an expansion of Spn polysaccharide–specific and total plasmablasts in blood. Moreover, increased responses of blood mucosa-associated invariant T (MAIT) cells against in vitro stimulation with pneumococcus prior to challenge associated with protection against establishment of Spn colonization and with increased mucosal MAIT cell populations. These results implicate MAIT cells in the protection against pneumococcal colonization and demonstrate that colonization affects mucosal and circulating B cell populations.
Source: Journal of Clinical Investigation - Category: Biomedical Science Authors: Source Type: research

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CONCLUSION: In the outpatient clinic specializing in oral anticoagulation, patients add more specific beliefs about the interference of vitamin K-rich diet on coagulation, when compared to those in the general cardiology outpatient clinic. PMID: 31618372 [PubMed - in process]
Source: Revista gaucha de enfermagem EENFUFRGS - Category: Nursing Tags: Rev Gaucha Enferm Source Type: research
CONCLUSION: The Tracer Methodology is used for evaluating and improving the quality of care. PMID: 31618371 [PubMed - in process]
Source: Revista gaucha de enfermagem EENFUFRGS - Category: Nursing Tags: Rev Gaucha Enferm Source Type: research
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Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
Publication date: Available online 16 October 2019Source: Ticks and Tick-borne DiseasesAuthor(s): Jinming Wang, Jifei Yang, Shandian Gao, Aihong Liu, Muhammad Rashid, Youquan Li, Zhijie Liu, Junlong Liu, Guangyuan Liu, Jianxun Luo, Guiquan Guan, Hong YinAbstractBovine theileriosis, caused by protozoan parasites of the genus Theileria, presents with various clinical symptoms. In cattle, clinical presentations and outcomes of bovine theileriosis are closely correlated with the causative Theileria spp. Thus, accurate detection and discrimination of Theileria spp. are essential for epidemiological studies and for provision of ...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
Publication date: Available online 16 October 2019Source: Small Ruminant ResearchAuthor(s): Alison M. Lee, Alan Wolfe, Joseph P. Cassidy, John Moriarty, Ronan O’Neill, Claire Fahy, Emily Connaghan, Chris Cousens, Mark P. Dagleish, Maire C. McElroy, Locksley L. McV. MessamAbstractJaagsiekte sheep retrovirus (JSRV) is the causative agent of ovine pulmonary adenocarcinoma (OPA). JSRV infection is usually detected post-mortem by macroscopic and histological examination of lungs for lesions of OPA. Subsequently, the presence of JSRV may be confirmed using polymerase chain reaction (PCR) on tumour tissue. Our goal was to d...
Source: Small Ruminant Research - Category: Zoology Source Type: research
This study not only represents the most systematic analysis of codon usage patterns in PEDV orf3 genes, but also provides a basic shaping mechanism of the codon usage bias.
Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research
Publication date: Available online 16 October 2019Source: Research in Veterinary ScienceAuthor(s): Baolin Song, Qianmei Zeng, Yuan Liu, Bangyuan WuAbstractAbundant evidence now supports the idea that methionine deficiency has negative effects on chicken healthy especially in the aspect of cell cycle regulation and apoptosis. But, lacking of knowledge is the evaluation on metabolic organs. To test the effect of methionine deficiency on the kidney, we assessed the apoptosis and the cell cycle of kidney induced by methionine deficiency by the methods of TdT-mediated dUTP Nick-End Labeling (TUNEL), flow cytometry (FCM), quanti...
Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research
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Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research
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Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research
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Source: Endocrinology - Category: Endocrinology Authors: Tags: Endocrinology Source Type: research
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