Canine multicentric lymphoma exhibits systemic and intratumoral cytokine dysregulation

Publication date: Available online 9 September 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): Joana N.R. Dias, Mariana Lopes, Conceição Peleteiro, Gonçalo Vicente, Telmo Nunes, Luísa Mateus, Frederico Aires-da-Silva, Luís Tavares, Solange GilAbstractNon-Hodgkin Lymphoma (NHL) is among the most common neoplasias in dogs and humans. Owing to remarkable similarities with its human counterpart, the canine lymphoma (cNHL) model has been proposed as a powerful framework for rapid and clinically relevant translation of novel immunotherapies. However, the establishment of cNHL as a predictive preclinical model has been hampered by the limited characterization of the canine immune system. Cytokines are key players of the interaction between tumor and its microenvironment. In human NHL, multiple cytokines have been linked to the development of lymphoma and are relevant biomarkers for treatment response and prognosis. In contrast, few studies have investigated cytokines in cNHL. Within this context, this study aimed to investigate cytokine regulation in cNHL.A multicentric cNHL biobank was successfully constructed. Cytokine mRNA profiles in tumor tissue and circulating PBMC were analyzed by qRT-PCR and compared to a healthy control group. Specific primers were used to evaluate Th1, Th2 and Th17 responses. Systemic cytokine concentrations were measured using a commercial canine multiplex assay which included IL-2, IL6, IL-10 and TNF-α, and compared to a healthy con...
Source: Veterinary Immunology and Immunopathology - Category: Veterinary Research Source Type: research