Urinary extracellular vesicles associated MCP-1 and NGAL derived from specific nephron segments differ between calcium oxalate stone formers and controls.

Urinary extracellular vesicles associated MCP-1 and NGAL derived from specific nephron segments differ between calcium oxalate stone formers and controls. Am J Physiol Renal Physiol. 2019 Aug 28;: Authors: Chirackal RS, Jayachandran M, Wang X, Edeh S, Haskic Z, Perinpam M, Halling TM, Mehta R, Rivera ME, Lieske JC Abstract Randall's plaque (RP, subepithelial calcification) appears to be an important precursor of kidney stone disease. However, RP cannot be noninvasively detected. The present study investigated candidate biomarkers associated with extracellular vesicles (EVs) in the urine of calcium stone formers (CSFs) with low (< 5% papillary surface area) and high (≥ 5% papillary surface area) percentage of RP and a group of non-stone formers. RP were quantitated via videotaping and image processing in consecutive CSFs undergoing percutaneous surgery for stone removal. Urinary EVs derived from cells of different nephron segments of CSFs (n=64) and non-stone formers (n=40) were quantified in bio-banked cell-free urine by standardized and validated digital flow cytometer using fluorophore-conjugated antibodies. Overall, the number of EVs carrying surface monocyte chemoattractant protein-1 (MCP-1) and neutrophil gelatinase-associated lipocalin (NGAL) were significantly lower in CSFs compared to non-stone former controls (p<0.05), but did not differ statistically between the CSFs with low and high RP. The number of EVs associate...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research