Photobiomodulation Enhances Cisplatin Cytotoxicity in a Culture Model with Oral Cell Lineages

This study investigated whether photobiomodulation (PBM) can potentiate the effects of cisplatin on keratinocytes (HaCat) and cancer cells (SCC25 and HN12).Cells were treated with laser (GaAlAs; 660nm; 60mW; 0.33J; 2.14W/cm2; 11.7J/cm2 and 6s) and cisplatin (7.8 µg/mL) to evaluate cell viability,Ki‐67, VEGF, TGF‐β1, EGF expression, and ROS production. Observations were validated in the SCC25 cell lineage, where the type of cell death (necrosis/apoptosis) and the amount of ATP were assessed. Cell lineages showed increased sensitivity to cisplatin assoc iated with PBM (Cis‐PBM). Ki‐67 was augmented in all cell lineages treated with Cis‐PBM when compared to cisplatin alone (Cis). Cis or Cis‐PBM significantly decreased VEGF expression in cancer cells, while no changes were seen in the expression of TGF‐β1 or EGF compared to control. ROS le vels were similar in the Cis and Cis‐PBM groups. Cells treated with Cis‐PBM died by apoptosis, leading to greater consumption of ATP. These observations suggest that PBM may potentiate the effects of cisplatin, leading to increased drug cytotoxicity and enhanced cell death.This article is protected by copyright. All rights reserved.

Source: Photochemistry and Photobiology - August 18, 2019 Category: Science Authors: Ivana M.A. Diniz, Giovanna R. Souto, Iuri D.P. Freitas, Jos é Alcides A. de Arruda, Janine M. da Silva, Tarcília A. Silva, Ricardo A. Mesquita Tags: Research Article Source Type: research