A novel, green cloud point extraction and separation of phenols and flavonoids from pomegranate peel: An optimization study using RCCD

Publication date: October 2019Source: Journal of Environmental Chemical Engineering, Volume 7, Issue 5Author(s): Pavankumar R. More, Shalini S. AryaAbstractCloud point extraction (CPE) is one of the novel; environment-friendly; energy; time and cost-effective extraction technique. Therefore, in the present work preconcentration and separation of bioactive compounds from pomegranate peel, a major waste of the pomegranate processing industry was carried out using CPE. CPE works on the principle of entrapping the hydrophobic bioactive compound in the micelle. Surfactant concentration (5–11%), pH (4–8), temperature (30–60 °C), and salt concentration (4–12%) optimization was carried out to separate total phenols and flavonoids with maximum recovery (%R), partition coefficient (Ks/a), fraction concentration (fc), and minimum loss (%L) using the rotatable central composite design (RCCD). The optimum levels were 8.22% surfactant (Triton X-114), 4% salt (NaCl) at temperature of 36.80 °C and pH 4 which resulted in 95% recovery, 14.59 Ks/a, 0.95 fc and 5.27% loss of total phenols with the maximum desirability about 0.846. While in the case of total flavonoids at 8.27% Triton X-114 at pH 5.07/34.30 °C and 4.06% NaCl resulted, 98% recovery, 9.71 Ks/a, 1.01 fc, and 2% loss with 0.842 desirability. The total yield obtained for total phenols was 205.2 mg of GAE/g while for total flavonoids it was 60.05 mg of QE/g of pomegranate peel powder. Therefore, it can be concluded...
Source: Journal of Environmental Chemical Engineering - Category: Chemistry Source Type: research