Purification of rabies virus produced in Vero cells grown in serum free medium.

Purification of rabies virus produced in Vero cells grown in serum free medium. Vaccine. 2019 Jul 09;: Authors: Trabelsi K, Ben Zakour M, Kallel H Abstract Rabies is a viral zoonosis caused by negative-stranded RNA viruses of the Lyssavirus genus. It can affect all mammals including humans. Dogs are the main source of human rabies deaths, contributing up to 99% of all rabies transmissions to humans. Vaccination against rabies is still the sole efficient way to fight against the disease. Cell culture vaccines are recommended by World Health Organization (WHO) for pre and post exposure prophylaxis; among them Vero cell rabies vaccines which are used worldwide. In this work we studied the purification of inactivated rabies virus produced in Vero cells grown in animal component free conditions, using different methods. Cells were grown in VP-SFM medium in stirred bioreactor, then infected at an MOI of 0.05 with the LP2061 rabies virus strain. Collected harvests were purified by zonal centrifugation, and by chromatography supports, namely the Capto Core 700 and the monolithic CIM-QA column. Generated data were compared in terms of residual DNA level, host cell proteins (HCP) level and the overall recovery yield. Rabies virus purification using the monolithic column resulted in the highest antigen recovery yield, equal to 94%. Capto Core 700 showed a lower yield, about 84%; whereas the purification yield by zonal centrifugation was equal to 60%. In terms of ho...
Source: Vaccine - Category: Allergy & Immunology Authors: Tags: Vaccine Source Type: research

Related Links:

Publication date: Available online 13 September 2019Source: Preventive Veterinary MedicineAuthor(s): Emily G. Hudson, Victoria J. Brookes, Salome Dürr, Michael P. WardAbstractAustralia is free of canine rabies, however northern regions ― such as the Northern Peninsula Area (NPA), Queensland ― are at risk of an incursion from nearby rabies-infected Indonesian islands. Early detection and reactive vaccination is the current Australian policy to eradicate an incursion. Early detection in this region is challenging, so pre-emptive vaccination might be an effective strategy. The NPA dog population also has a heterogeno...
Source: Preventive Veterinary Medicine - Category: Veterinary Research Source Type: research
CONCLUSION: This study concludes that respondents have relatively limited knowledge, inconsistent attitude and poor practices towards Rabies prevention and control. There is a high need of imparting knowledge to the population from multidisciplinary programs for effective management and prevention of Rabies. PMID: 31491398 [PubMed - as supplied by publisher]
Source: Acta Tropica - Category: Infectious Diseases Authors: Tags: Acta Trop Source Type: research
uster Vos Finke Müller : To evaluate the long-term immunogenicity of the live-attenuated, oral rabies vaccine SPBN GASGAS in a full good clinical practice (GCP) compliant study, forty-six (46) healthy, seronegative red foxes (Vulpes vulpes) were allocated to two treatment groups: group 1 (n = 31) received a vaccine bait containing 1.7 ml of the vaccine of minimum potency (106.6 FFU/mL) and group 2 (n = 15) received a placebo-bait. In total, 29 animals of group 1 and 14 animals of group 2 were challenged at 12 months post-vaccination with a fox rabies virus isolate (103.0 MICLD50/mL). While 90% of the anim...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research
IJERPH, Vol. 16, Pages 2816: Ecological and Epidemiological Findings Associated with Zoonotic Rabies Outbreaks and Control in Moshi, Tanzania, 2017–2018 International Journal of Environmental Research and Public Health doi: 10.3390/ijerph16162816 Authors: Niwael Mtui-Malamsha Raphael Sallu Gladys R. Mahiti Hussein Mohamed Moses OleNeselle Bachana Rubegwa Emmanuel S. Swai Selemani Makungu Edward G. Otieno Athuman M. Lupindu Erick Komba Robinson Mdegela Justine A. Assenga Jubilate Bernard Walter Marandu James Warioba Zacharia Makondo Jelly Chang’a Furaha Mramba Hezron Non...
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Article Source Type: research
This study evaluated: (i) whether SINAN can be reliably used for rabies surveillance; (ii) if patients in Brazil are receiving appropriate PEP and (iii) the benefits of implementing the latest Wo rld Health Organization (WHO) recommendations on PEP. Analysing SINAN records from 2008 to 2017 reveals an average of 506,148 bite-injury patients/year [range: 437k -545k] in the country, equivalent to an incidence of 255 bite-injuries/100,000 people/year [range: 231–280]. The number of reports of bites from suspect rabid dogs generally increased over time. In most states, records from SINAN indicating a suspect rabid dog do...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
The virus has been defeated in many parts of the world. But where it persists, rabies takes a particular toll on the rural poor.
Source: NYT - Category: American Health Authors: Tags: Rabies Dogs Vaccination and Immunization World Health Organization World Organization for Animal Health (OIE) Food and Agriculture Organization of the United Nations Mission Rabies Humane Society International India your-feed-science Source Type: news
[Nyasa Times] Lilongwe Society for the Protection and Care of Animals (LSPCA) has created action groups to conduct free rabies vaccination in the city to curb prevalence of rabies cases and speculations.
Source: AllAfrica News: Health and Medicine - Category: African Health Source Type: news
Abstract Rabies is a neglected disease with an estimated annual mortality of 55,000 human deaths, affecting mainly low-income countries. Over 95% of these cases result from virus transmission through the bite of infected dogs and for this reason there is a real need for a cheap and effective rabies veterinary vaccine to be used in mass vaccination campaigns. In this work, we describe the establishment of a simple platform for the production of a virus-like particles based rabies vaccine using mammalian cells and roller bottles as culture system. Adherent cells were cultured during more than 15 days and VLPs were c...
Source: Comparative immunology, microbiology and infectious diseases. - Category: Allergy & Immunology Authors: Tags: Comp Immunol Microbiol Infect Dis Source Type: research
Rabies is one of the world's major zoonoses. Controlling rabies continues to pose a major public health challenge. The issues surrounding dog bites and the vaccination of dogs against rabies are important to consider in implementing programmes to control t...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Economics of Injury and Safety, PTSD, Injury Outcomes Source Type: news
In this study, we developed an adjuvanted hydrogel-based pDNA nanoparticulate vaccine for rabies protection and immunocontraception. In vivo, we observed an immune response skewed toward a Th2 type, in contrast to the Th1 type in our previous pDNA study. The observation was verified by the IgG2a/IgG1 ratio (
Source: Nanomedicine: Nanotechnology, Biology and Medicine - Category: Nanotechnology Source Type: research
More News: Allergy & Immunology | International Medicine & Public Health | Rabies | Science | Vaccines | Veterinary Vaccinations | WHO