Chemical Profile and In Vivo Toxicity Evaluation of Unripe Citrus Aurantifolia Essential Oil

Publication date: Available online 12 July 2019Source: Toxicology ReportsAuthor(s): Christian K. Adokoh, Du-Bois Asante, Desmond O. Acheampong, Yohei Kotsuchibashi, Francis A. Armah, Ignatius H. Sirikyi, Keisuke Kimura, Edward Gmakame, Sey Abdul-RaufAbstractCitrus aurantifolia (Christm.) Swingle (syn. C. MEDICA var. ACIDA Brandis) (family: Rutaceae) essential oil is one of the local and cheapest oil found in the market. Although, it is generally accepted as non-toxic to vital organs and cells, majority of people are cynical about it usage. Herein, the present paper reports the chemical composition and in vivo oral toxicity study of unripe C. aurantifolia essential oil found in Ghana. The toxicity of C. aurantifolia essential oil extract was investigated via oral administration using two methods: The acute toxicity single dose study (SDS) and the repeated dose method. The oil exhibited no acute toxicity but in the sub-chronic studies, the effects was dose and time-dependent. Chemical profile investigation of the oil showed 9 constituent of phytochemicals (Germacrene isomers (61.2%), Pineen (14%), Linalool dimmer (2.9%), Bornane (11%), Citral (2.9%), Anethole (1.5%), Anisole (1.1%), Safrole (0.3%) and Demitol (0.6%)). Histopathological studies revealed conditions such as necrosis, edema and inflammatory reaction in the liver, spleen and kidneys. Marginal upsurge of biochemical parameters above normal and elevated levels of lymphocytes (35.20 to 46.40 g/dL) demonstrated mild t...
Source: Toxicology Reports - Category: Toxicology Source Type: research