Isolation, Purification, and Culture of Mouse Pancreatic Islets of Langerhans.

Isolation, Purification, and Culture of Mouse Pancreatic Islets of Langerhans.
Methods Mol Biol. 2019;1940:255-265
Authors: Saliba Y, Farès N
Abstract
Pancreatic islets constitute an important tool for research and clinical applications in the field of diabetes. They are used for transplantation, unraveling new mechanisms in insulin secretion, studying pathophysiological pathways in diseased cells, and pharmacological research aimed at developing improved therapeutic strategies. Therefore, fine-tuning islet isolation protocols remains an important objective for reliable investigations. Here we describe a relatively simple mouse islet isolation protocol that relies on enzymatic digestion using low-activity collagenase and several sedimentation and Percoll gradient steps.
PMID: 30788831 [PubMed - indexed for MEDLINE]

Source: Mol Biol Cell - July 13, 2019 Category: Molecular Biology Authors: Saliba Y, Farès N Tags: Methods Mol Biol Source Type: research