Molecules, Vol. 24, Pages 2549: Velutin, an Aglycone Extracted from Korean Mistletoe, with Improved Inhibitory Activity against Melanin Biosynthesis
Molecules, Vol. 24, Pages 2549: Velutin, an Aglycone Extracted from Korean Mistletoe, with Improved Inhibitory Activity against Melanin Biosynthesis
Molecules doi: 10.3390/molecules24142549
Authors:
Se-Hui Jung
Jaehyun Kim
Juneyong Eum
Jung Won Choe
Hak Hyun Kim
Yun Kee
Kooyeon Lee
The abnormal regulation of melanin synthesis leads to a wide range of pigmentary disorders. Although various melanin biosynthesis inhibitors have been developed, their efficacy and long-term safety needs to be further improved, and thus the goal of this study is to develop promising natural compound inhibitors of melanin biosynthesis. Here, we obtained aglycone flavonoid extract through the microwave-assisted hydrolysis of glycoside extract from Korean mistletoe in acidic condition. The aglycone extract inhibited tyrosinase activity more efficiently with better antioxidant activity than glycoside extract in vitro. The microwave-assisted aglycone extract of mistletoe was further analyzed for in vivo activity, and the results showed the aglycone extract inhibited both early melanocyte development and melanin synthesis more efficiently in zebrafish embryo in a dose-dependent manner. Our in vivo toxicity assay quantitatively measured cell death in zebrafish embryos and showed that the microwave-assisted aglycone extract of mistletoe had no significant effect on cell death (p < 0.001), indicating that aglycone extract is more biocompatible than glycoside extract. Furthermo...
Source: Molecules - Category: Chemistry Authors: Se-Hui Jung Jaehyun Kim Juneyong Eum Jung Won Choe Hak Hyun Kim Yun Kee Kooyeon Lee Tags: Article Source Type: research