Development of a dosimetric model for < i > in vitro < /i > labelled cells with β+ emitters in PET tracking studies.

Development of a dosimetric model for <i>in vitro</i> labelled cells with β+ emitters in PET tracking studies.
Phys Med Biol. 2019 Jun 26;:
Authors: Jacquemin M, Broggio D, Franck D, Desbree A
Abstract
Positron emission tomography (PET) offers an effective method for tracking β+ emitters-labeled cells in vivo. However, in vitro high labelling activities used may cause cell damage or death. Our understanding of the impact of such procedure remains limited by the fact that the biological effects are usually linked to the activity per cell rather than the absorbed dose. To assess the dose delivered to the cells during the radiolabelling, a multi-cellular dosimetry computational tool was developed, allowing the study of two key parameters: the cell density and the labelling efficiency. Through a hybrid method based on Monte Carlo simulations (MCNP6 code) and an analytical approach implemented in Python, the mean absorbed dose received by a target cell was efficiently calculated for distributions with a very large number of cells - up to hundreds of millions. An advanced investigation of in vitro cell labelling with β-emitting radionuclides was carried out via (i) a systematic study of the effects of the labelling parameters on the cell absorbed dose for 18F, 64Cu and 68Ga, and (ii) a quantitative comparison between cellular and conventional dosimetry. The results provided a thorough analysis of how the dose (self, cross an...

Source: Physics in Medicine and Biology - June 25, 2019 Category: Physics Authors: Jacquemin M, Broggio D, Franck D, Desbree A Tags: Phys Med Biol Source Type: research