Structural characterization and anticoagulant activity of two polysaccharides from Patinopecten yessoensis viscera.

Structural characterization and anticoagulant activity of two polysaccharides from Patinopecten yessoensis viscera. Int J Biol Macromol. 2019 Jun 17;: Authors: Song S, Wang L, Wang L, Yu Q, Ai C, Fu Y, Yan C, Wen C, Zhu Z Abstract In the present study, two polysaccharides, SVP2-1 and SVP2-2, were isolated from Patinopecten yessoensis viscera and purified by using DEAE-52 cellulose and Sepharose CL-6B. Both SVP2-1 and SVP2-2 could extend activated partial thromboplastin time (APTT) and thrombin time (TT) and inhibit the transformation of fibrinogen into fibrin (FIB) concentration-dependently, indicating they inhibited clotting and thrombin through intrinsic and common pathways. Of note, SVP2-2 had stronger anticoagulant activity than SVP2-1, and its backbone was determined as →6)-α-Manp (1 → 2)-α-Galp(1 → with Xyl or Glc substituted at C4 of Gal. Based on monosaccharide composition analysis, methylation analysis, and NMR analysis. Further comparison of their monosaccharide analysis and NMR spectra indicates SVP2-1 and SVP2-2 possess the same core structure features, so the higher sulfate content and lower molecular weight may be the possible reasons for the stronger anticoagulant capability of SVP2-2. The present study suggests acidic polysaccharides from scallop viscera as promising anticoagulant candidates. PMID: 31220498 [PubMed - as supplied by publisher]
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research