Embryonic or Induced Pluripotent Stem Cell-Derived Hepatocellular Systems for HCV Culture.

Embryonic or Induced Pluripotent Stem Cell-Derived Hepatocellular Systems for HCV Culture.
Methods Mol Biol. 2019;1911:121-135
Authors: Wu X, Dao Thi VL
Abstract
Human-induced pluripotent stem cell-derived hepatocyte-like cells (iHeps) constitute a powerful tool for modeling hepatotropic pathogen infections in cell culture. Meanwhile, CRISPR-Cas9 technology enables precise editing of stem cell genomes to generate patient-specific disease models and thus development of personalized experimental systems. Here we present a detailed stepwise protocol for the differentiation of stem cells to hepatocyte-like cells for HCV studies in cell culture. We also outline the use of an inducible iCRISPR platform for the rapid and efficient modification of host factors of interest to better understand their function during HCV infection.
PMID: 30593622 [PubMed - indexed for MEDLINE]

Source: Mol Biol Cell - June 20, 2019 Category: Molecular Biology Authors: Wu X, Dao Thi VL Tags: Methods Mol Biol Source Type: research