Generation of Xenopus Haploid, Triploid, and Hybrid Embryos.

Generation of Xenopus Haploid, Triploid, and Hybrid Embryos.
Methods Mol Biol. 2019;1920:303-315
Authors: Gibeaux R, Heald R
Abstract
Frog species of the genus Xenopus are widely used for studies of cell and developmental biology, and recent genome sequencing has revealed interesting phylogenetic relationships. Here we describe methods to generate haploid, triploid, and hybrid species starting from eggs and sperm of Xenopus laevis and Xenopus tropicalis that enable investigation of how genome size and content affect physiology at the organismal, cellular, and subcellular levels.
PMID: 30737699 [PubMed - indexed for MEDLINE]

Source: Mol Biol Cell - June 20, 2019 Category: Molecular Biology Authors: Gibeaux R, Heald R Tags: Methods Mol Biol Source Type: research