Optimized Protocol of Zebrafish Somatic Cell Nuclear Transfer (SCNT).
Optimized Protocol of Zebrafish Somatic Cell Nuclear Transfer (SCNT).
Methods Mol Biol. 2019;1920:353-375
Authors: Prukudom S, Siripattarapravat K, Poulos W, Cibelli JB
Abstract
Zebrafish (Danio rerio) is an established animal model to study developmental biology as well as a wide array of human diseases. Here we describe a protocol for somatic cell nuclear transfer (SCNT). This protocol can be used to introduce genetic modifications in zebrafish and for the study of cell plasticity.
PMID: 30737703 [PubMed - indexed for MEDLINE]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Prukudom S, Siripattarapravat K, Poulos W, Cibelli JB Tags: Methods Mol Biol Source Type: research