Influence of renal ischemia-reperfusion injury on renal neutrophil gelatinase- associated lipocalin receptor (24p3R) in rats.

This study was undertaken to evaluate an influence of renal ischemia-reperfusion injury on 24p3R. Unilateral renal pedicle was clamped for 0, 10, 20, 30, or 45 min in male Wistar rats. Urine was collected for 24 hours after reperfusion, and ischemic kidney and blood sample were obtained. Apparent histological injury in the ischemic kidney was detected in the 30min- and 45 min-treated groups. Urinary NGAL excretion elevated in rats with renal ischemia for more than 20 min, while serum creatinine increased in rats for more than 30 min of ischemia. Renal protein expression of NGAL did not significantly change. Renal mRNA expressions of megalin and cubilin, which are expressed at renal proximal tubules and uptake NGAL, decreased in animals with renal ischemia for more than 20 min. Renal protein expression of 24p3R, which is expressed at renal distal tubules and uptake NGAL, decreased in rats with renal ischemia for 45 min. This study showed for the first time that renal 24p3R decreased in response to renal ischemia. As relatively longer renal ischemia (45 min) decreased renal 24p3R protein and increased urinary NGAL excretion, the down-regulation of 24p3R protein might contribute to the elevated urinary excretion of NGAL in rats with unilateral ischemia-reperfusion injury. This article is protected by copyright. All rights reserved. PMID: 31211866 [PubMed - as supplied by publisher]
Source: Clinical and Experimental Pharmacology and Physiology - Category: Drugs & Pharmacology Authors: Tags: Clin Exp Pharmacol Physiol Source Type: research