Ionic liquid-based miniaturized aqueous biphasic system to develop an environmental-friendly analytical preconcentration method.

Ionic liquid-based miniaturized aqueous biphasic system to develop an environmental-friendly analytical preconcentration method. Talanta. 2019 Oct 01;203:305-313 Authors: González-Martín R, Pacheco-Fernández I, Ayala JH, Afonso AM, Pino V Abstract Two ILs containing guanidinium cations (butylguanidinium chloride -C4Gu-Cl- and hexylguanidinium chloride -C6Gu-Cl-) were synthesized and characterized. Their cytotoxicity was also assessed, obtaining adequate CC50 values of 680 ± 99 mg·L-1 for C4Gu-Cl and 135 ± 8 mg·L-1 for C6Gu-Cl. Miniaturized aqueous biphasic systems (μ-ABSs) were developed using amounts lower than 1% (w/w) of these synthesized guanidinium-based ILs, K3PO4 as salting-out agent, and ultrapure water. The phases diagrams of both systems were determined, and the C4Gu-Cl-based μ-ABS was selected for the development of a microextraction method in combination with high performance liquid chromatography (HPLC) with fluorescence detection (FD) for the determination of five polycyclic aromatic hydrocarbons (PAHs) as model analytes. A point of the biphasic region of the C4Gu-Cl-based μ-ABS corresponding to a mixture of 0.75% (w/w) of the IL, 37.7% (w/w) of K3PO4 and 61.55% (w/w) of ultrapure water, and 30 min of equilibrium time, were selected as optimum conditions to obtain high enrichment factors and proper analytical microextraction performance. The C4Gu-Cl-based μ-ABS-HPLC-FD method exhibited low limit...
Source: Talanta - Category: Chemistry Authors: Tags: Talanta Source Type: research