GSE132729 Cap analysis gene expression (CAGE) of Aspergillus luchuensis mut. kawachii control and sirtuin D disruptant strains

In this study, we characterized sirtuin in the white koji fungus, Aspergillus kawachii, to examine their role on regulation of amylolytic enzymes and citric acid productions during the solid-state culture (koji). Characterization of rice-koji made using five putative sirtuin gene disruptants indicated that they are involved in amylolytic activity and acidity of rice koji; especially the sirD disruptant showed lower levels of α-amylase activity and citric acid production per mycelial weight in the koji compared the control strain. In addition, the sirD disruptant also showed a change in mycelial pigmentation, higher sensitivity to cell wall biogenesis inhibitor such as calcofluor white and Congo red, and reduced conidia formation, indicating that SirD is required for secondary metabolism, cell wall integrity, and conidial development. The cap analysis gene expression (CAGE) indicated that the transcriptional changes related to the characteristic phenotype of the sirD disruptant (e.g., a reduced transcript level of acid-stable α-amylase gene and a citric acid exporter) in rice koji. These results indicated that SirD has a significant role on the global transcriptional regulation including productions of α-amylase and citric acid in A. kawachii during the solid-state fermentation process.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Aspergillus kawachii Source Type: research

Related Links:

Tue, 07/16/2019 - 14:41News blog
Source: The Aspergillus Website - updates - Category: Respiratory Medicine Authors: Source Type: news
ABSTRACT Re-engineering of chemical materials at the nanoscale level that employ modification and improvement in their physical and chemical properties has been constantly pursued for application in biomedical and biotechnology industries. Moreover, immobilization of catalysts on these bio/chemically modified nanomaterials improved the performance of enzymes in a plethora of industrial uses. Hence, in this study, cerium oxide nanoparticles (CNPs) were synthesized and their morphology was investigated by TEM and UV-spectra. They were modified by carboxylation and glutaraldehyde to achieve highly efficient surface functional...
Source: Brazilian Journal of Chemical Engineering - Category: Chemistry Source Type: research
Lucia Caputo There is a growing interest in a potential use of essential oils (EOs) as a replacement for traditional pesticides and herbicides. The aims of this study were to: (i) Identify the chemical composition of the two EOs derived from Origanum heracleoticum L. and O. majorana L., (ii) evaluate the in vitro antifungal activity of the EOs against some postharvest phytopathogens (Botrytis cinerea, Penicillium expansum, Aspergillus niger and Monilinia fructicola), (iii) evaluate the in vitro antibacterial activity against Bacillus megaterium, Clavibacter michiganensis, Xanthomonas campestris, Pseudomonas fluoresc...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
Aspergillus fumigatus and A. flavus are fungal pathogens linked to most cases of invasive aspergillosis (IA). Early detection of the circulating antigen galactomannan (GM) in serum using specific antibodies allows the prompt application of effective antifungal therapy, thus improving the survival rate of IA patients. The use of known mAbs in the IA diagnosis is often associated with false positive results due to cross-reactivity with some bacterial polysaccharides , that challenge the search of more specific mAbs. Here we describe the characterization of the Aspergillus-specific monoclonal antibody (mAb) AP3 (IgG1κ),...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
In this study the effects against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Acinetobacterbaumannii, Streptococcus pyogenes, Proteus vulgaris, Salmonella typhi, Shigella spp., Candida albicans, Candida parapsilosis, Anopheles stephensi, Aedes aegypti, A. aegypti, A. albopictus, Culex pipiens, Trypanosoma brucei, Leishmania major, Trichomonas. Vaginalis, poliovirus type 1, coxsackie virus B, echovirus 6, West Nile Virus, herpes virus type 1, HSV-1 virus, Fusarium spp., Aspergillus flavus, Aspergillus niger, C. albicans, Alternaria alternata, Setosphaeria turcica and Magnaporthe grisea was revealed. ...
Source: Comparative immunology, microbiology and infectious diseases. - Category: Allergy & Immunology Authors: Tags: Comp Immunol Microbiol Infect Dis Source Type: research
Publication date: Available online 13 July 2019Source: Current Opinion in Food ScienceAuthor(s): Cathrine Finne Kure, Ida SkaarMould growth on cheese represents both a quality and a food safety problem, and poses significant economic losses. Several mould genera may destroy cheese, however normally just a few fungal species dominate on a specific type of cheese. Penicillium is the major genus followed by Aspergillus. Cheese contaminating mould species may produce mycotoxins, and some of the toxins as ochratoxin A, cyclopiazonic acid and sterigmatocystin have been shown to be stable under normal processing conditions. The m...
Source: Current Opinion in Food Science - Category: Food Science Source Type: research
In conclusion, EVs of ASCs ameliorated Th2-mediated inflammation induced by Aspergillus protease antigen through the activation of dendritic cells and M2 macrophage, accompanied by down-regulation of eotaxin and IL-25, and up-regulation of TGF-β and IL-10 in mouse lung epithelial cells.
Source: Stem Cell Research - Category: Stem Cells Source Type: research
Publication date: Available online 12 July 2019Source: Enzyme and Microbial TechnologyAuthor(s): Pornpimol Phuengmaung, Yoichi Sunagawa, Yosuke Makino, Takafumi Kusumoto, Satoshi Handa, Wasana Sukhumsirichart, Tatsuji SakamotoAbstractWe previously described the fungus Penicillium chrysogenum 31B, which has high performance to produce the ferulic acid esterase (FAE) for de-esterifying ferulic acids (FAs) from sugar beet pulp. However, the characteristics of this fungus have not yet been determined. Therefore, in this study, we evaluated the molecular characteristics and natural substrate specificity of the Pcfae1 gene from ...
Source: Enzyme and Microbial Technology - Category: Biotechnology Source Type: research
Publication date: Available online 12 July 2019Source: Journal of Molecular BiologyAuthor(s): Georgia F. Papadaki, George Lambrinidis, Andreas Zamanos, Emmanuel Mikros, George DiallinasAbstractFurE, a member of the NCS1 family, is an Aspergillus nidulans transporter specific for uracil, allantoin and uric acid. Recently, we showed that C- or N-terminally truncated FurE versions are blocked for endocytosis and, surprisingly, show modified substrate specificities. Bifluorescence complementation assays and genetic analyses supported the idea that C- and N-termini interact dynamically and through this interaction regulate sele...
Source: Journal of Molecular Biology - Category: Molecular Biology Source Type: research
In conclusion, these results suggest that AuNPsQct, produced by cost-effective method, can act as a promising candidate for different medical applications.
Source: Saudi Pharmaceutical Journal - Category: Drugs & Pharmacology Source Type: research
More News: Aspergillus | Congo Health | Genetics | Study