Optimization of process variables for enhanced production of urease by indigenous Aspergillus niger strains through response surface methodology

Publication date: Available online 13 June 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Yasir Mehmood Khan, Hira Munir, Zahid AnwarAbstractUrease is one of the important enzymes secreted by microorganisms in the soil, but can also be produced by microbial fermentation. The present study aimed to explore optimum conditions for maximum urease enzyme production from wheat straw by applying a statistical approach, response surface methodology (RSM), under the solid-state fermentation process. Eight different local strains of Aspergillus niger were screened for the production of urease enzyme. Fermentation profile revealed that all the strains showed activity but two strains BMB-02 NSJ and BMB-05 G displayed the higher activity as compared to the other strains. Therefore, two strains explicitly BMB-02 NSJ and BMB-05 G were shortlisted for further optimization studies. Different process parameters including incubation time, temperature, and pH were optimized for urease production. The result showed that both strains of A. niger (BMB0-02 NSJ and BMB-05 G) displayed maximum enzyme activity at 144 h (6 days), 35 °C, and pH 9.0, respectively. Moreover, RSM was employed to optimize the different parameters such as biomass, inoculum size, nitrogen content and moisture for both strains. Optimal enzyme conditions for BMB-02 NSJ were biomass, inoculum size, nitrogen, and moisture of 10 g, 5 mL, 0.5 g and 250% ratio to biomass, respectively, whereas b...
Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research

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Publication date: Available online 19 July 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Hafsa Sattar, Zainab Bibi, Aysha Kamran, Afsheen Aman, Shah Ali Ul QaderAbstractSerine metalloproteases have substantial scientific interest due to their unique physicochemical properties and capability to degrade complex casein polymer. In this contemporary study, the production of protease was enhanced by optimizing various physicochemical parameters and one-variable at a time approach used under submerged fermentation conditions. Among four different previously isolated Aspergillus species, Aspergillus niger KIBG...
Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research
Wei-Zhong Liu Three new γ-hydroxyl butenolides (1–3), a pair of new enantiomeric spiro-butenolides (4a and 4b), a pair of enantiomeric cyclopentenones (5a new and 5b new natural), and six known compounds (6–11), were isolated from Aspergillus sclerotiorum. Their structures were established by spectroscopic data and electronic circular dichroism (ECD) spectra. Two pairs of enantiomers [(+)/(–)-6c and (+)/(–)-6d] obtained from the reaction of 6 with acetyl chloride (AcCl) confirmed that 6 was a mixture of two pairs of enantiomers. In addition, the X-ray data confirmed tha...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
Publication date: Available online 19 July 2019Source: Microbial PathogenesisAuthor(s): Tina Nasrin, Mousumi Patra, Mauricio Escudey, Tapan Kumar DasAbstractAimsE. coli is a widely known model organism for life science research, especially in modern bio-engineering and industrial microbiology. The goal of our current study is to understand the growth inhibitory mechanism of biosynthesized CdS nanoparticles on E. coli bacteria.Main methodsCharacterization of Aspergillus foetidus mediated CdS nanoparticles has been confirmed by Zeta potential, AFM and HRTEM analyses. Furthermore, we investigated the contribution of reactive ...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
Publication date: Available online 19 July 2019Source: Journal of Clinical NeuroscienceAuthor(s): Che-Wei Hsu, Wan-Chen Tsai, Chia-Yi Lien, Jun-Jun Lee, Wen-Neng ChangAbstractIn this magnetic resonance imaging-based study, we investigated the clinical features, neuroimaging features and therapeutic outcomes of 14 adults (eight men and six women; mean age 60.4 years; range 37–77 years) with septic cavernous sinus thrombosis (CST). Of the underlying conditions, 10 had diabetes mellitus and 13 had concomitant sphenoid sinusitis. Headache (n = 13) and ophthalmoplegia (n = 13) were the most common clinical pre...
Source: Journal of Clinical Neuroscience - Category: Neuroscience Source Type: research
Publication date: Available online 18 July 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): M. Shanmugavel, J. Nivedha lakshmi, S. Vasantharaj, C. Anu, L. Edwin Paul, R. Praveen Kumar, A. GnanamaniAbstractIn the present study, the Aspergillus flavus were screened to produce multienzyme containing amylase and protease using starch agar and skimmed milk agar plate assay. The fungus was cultivated by solid state fermentation using wheat bran as substrate. The parameters such as pH, temperature, incubation time, and moisture content were optimized. The maximum production was achieved by amylase with a yield of...
Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research
Publication date: Available online 19 July 2019Source: Food ControlAuthor(s): Elizabeth C. Buerman, Randy W. Worobo, Olga I. Padilla-ZakourAbstractHigh Pressure Processing (HPP) is a method used to extend shelf life of foods by subjecting products in their final packaging to extreme pressure. This leads to protein degradation in microbial cells, membrane degradation, and eventual cell death. Survival of spoilage organisms, especially spore-formers, has not been closely considered in regard to the effect of water activity (aw). The purpose of this study was to determine the effect of pH and aw on HPP apple juice to prevent ...
Source: Food Control - Category: Food Science Source Type: research
ConclusionThe findings from these investigations could be used for future cultivation of A. terreus in the production of desired metabolites.
Source: Journal of Microbiological Methods - Category: Microbiology Source Type: research
CONCLUSIONS: Surgery is needed in some patients with refractory CPA. We consider that SAIA and CCPA patients may benefit from perioperative antifungal therapy. However, due to the limited number of patients in this study, more evidence is needed to draw a conclusion about this issue. PMID: 31312378 [PubMed]
Source: American Journal of Translational Research - Category: Research Tags: Am J Transl Res Source Type: research
Publication date: Available online 19 July 2019Source: SteroidsAuthor(s): Temitayo O. Ajayi, Radhakrishnan Srivedavyasasri, Emmanuel E. Nyong, Micheal A. Odeniyi, Jones O. Moody, Samir A RossAbstractThe crude methanol extract of Sphenocentrum jollyanum root exhibited 98% and 80% antimicrobial activity against Aspergillus fumigatus Pinh and Vancomycin resistant enterococcus (VRE) at a concentration of 200 µg/mL, with IC50 11.45 and 12.95 µg/mL, respectively. The ethyl acetate fraction of methanol extract showed in-vitro antimicrobial activity against A. fumigatus Pinh at 83% with IC50 of
Source: Steroids - Category: Drugs & Pharmacology Source Type: research
Publication date: Available online 17 July 2019Source: Enzyme and Microbial TechnologyAuthor(s): Siti Intan Rosdianah Damis, Abdul Munir Abdul Murad, Farah Diba Abu Bakar, Siti Aishah Rashid, Nardiah Rizwana Jaafar, Rosli Md. IlliasAbstractEnzyme hydrolysis faces a bottleneck due to the recalcitrance of the lignocellulose biomass. The protein engineering of GH11 xylanase from Aspergillus fumigatus RT-1 was performed near the active site and at the N-terminal region to improve its catalytic efficiency towards pretreated kenaf (Hibiscus cannabinus) hydrolysis. Five mutants were constructed by combined approaches of error-pro...
Source: Enzyme and Microbial Technology - Category: Biotechnology Source Type: research
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