Bioevaluation of 99mTc(I) Carbonyl-Radiolabeled aminoacid coated magnetic nanoparticles in vivo

Publication date: Available online 13 June 2019Source: Materials Chemistry and PhysicsAuthor(s): Onur Büyükok, Eser Uçar, Çiğdem İçhedef, Oğuz Çetin, Serap TeksözAbstractIn this paper, amino acid coated and radiolabeled magnetic nanoparticles (MNPs) were prepared as a potential diagnostic agent. Magnetic nanoparticles were synthesized and functionalized by L-alanine and L-cysteine amino acids in one step. Besides surface functionalization, amino acid coating also provided favorable particle size. Structural characterization was performed by scanning electron microscopy. We obtained 30-38nm and 22-30 nm diameter ranges with spherical shape for L-alanine and L-cysteine coated nanoparticles, separately. Then L-alanine and L-cysteine coated MNPs were radiolabeled with 99mTc(I)-tricarbonyl core (99mTc(CO)3-Alanine-MNP, 99mTc(CO)3-Cysteine-MNP) with a labeling yield of 63±3% and 74±2%. Pure radiolabeled magnetic particles were obtained by washing them with saline solution, and the radiochemical purity of 99mTc(CO)3-Alanine-MNP and 99mTc(CO)3-Cysteine-MNP were found as 94.3±0.47% and 97.6±1.06% in each of the final solution, respectively. In addition, both radiolabeled nanoparticles were stabile up to 4 hours at 37°C temperature in vitro conditions.
Source: Materials Chemistry and Physics - Category: Materials Science Source Type: research