The biochemistry surrounding bovine conceptus elongation.

The biochemistry surrounding bovine conceptus elongation. Biol Reprod. 2019 Jun 10;: Authors: Simintiras CA, Sánchez JM, McDonald M, Lonergan P Abstract Conceptus elongation is a fundamental developmental event coinciding with a period of significant pregnancy loss in cattle. The process has yet to be recapitulated in vitro whereas in vivo it is directly driven by uterine secretions and indirectly influenced by systemic progesterone. To better understand the environment facilitating this critical reproductive phenomenon, we interrogated the biochemical composition of uterine luminal fluid from heifers with high vs. physiological circulating progesterone on Days 12-14 of the estrous cycle-the window of conceptus elongation-initiation-by high-throughput untargeted ultra-high-performance liquid chromatography tandem mass spectroscopy. A total of 233 biochemicals were identified, clustering within 8 super-pathways [amino acids (33.9%), lipids (32.2%), carbohydrates (8.6%), nucleotides (8.2%), xenobiotics (6.4%), cofactors and vitamins (5.2%), energy substrates (4.7%), and peptides (0.9%)] and spanning 66 metabolic sub-pathways. Lipids dominated total progesterone (39.1%) and day (57.1%) effects; however, amino acids (48.5%) and nucleotides (14.8%) accounted for most day by progesterone interactions. Corresponding pathways over-represented in response to day and progesterone include (i) methionine, cysteine, s-adenosylmethionine, and tau...
Source: Reproductive Biology - Category: Reproduction Medicine Authors: Tags: Biol Reprod Source Type: research