Hc TTR: a novel antagonist against goat interleukin 4 derived from the excretory and secretory products of Haemonchus contortus

In this study, three proteins, glutathioneS-transferase domain containing protein (HcGST), transthyretin domain containing protein (HcTTR) and calponin actin-binding domain containing protein (HcCab), were identified to bind to goat IL4 by co-immunoprecipitation (Co-IP) assays and yeast two-hybrid screening. Additionally, cell proliferation analysis showed thatHcTTR blocked the IL4-induced proliferation of peripheral blood mononuclear cells in goats, whileHcGST andHcCab did not. In addition,HcTTR could also downregulate the transcription of candidate genes in the IL4-induced JAK/STAT pathway. These results indicated thatHcTTR is a novel antagonist against goat IL4 fromHcESPs, and this information could improve our understanding of the relationship between host cytokines and parasite infections.
Source: Veterinary Research - Category: Veterinary Research Source Type: research