Intermolecular Interactions between Hsp90 and Hsp70

Publication date: Available online 22 May 2019Source: Journal of Molecular BiologyAuthor(s): Shannon M. Doyle, Joel R. Hoskins, Andrea N. Kravats, Audrey L. Heffner, Srilakshmi Garikapati, Sue WicknerAbstractMembers of the Hsp90 and Hsp70 families of molecular chaperones are important for the maintenance of protein homeostasis and cellular recovery following environmental stresses, such as heat and oxidative stress. Moreover, the two chaperones can collaborate in protein remodeling and activation. In higher eukaryotes, Hsp90 and Hsp70 form a functionally active complex with Hop (Hsp90-Hsp70 organizing protein) acting as a bridge between the two chaperones. In bacteria, which do not contain a Hop homolog, Hsp90 and Hsp70, DnaK, directly interact during protein remodeling. Although yeast possess a Hop-like protein, Sti1, Hsp90 and Hsp70 can directly interact in yeast in the absence of Sti1. Previous studies showed that residues in the middle domain of Escherichia coli Hsp90 are important for interaction with the J-protein binding region of DnaK. The results did not distinguish between the possibility that i) these sites were involved in direct interaction and ii) the residues in these sites participate in conformational changes which are transduced to other sites on Hsp90 and DnaK that are involved in the direct interaction. Here we show by crosslinking experiments that the direct interaction is between a site in the middle domain of Hsp90 and the J-protein binding site of Hsp7...
Source: Journal of Molecular Biology - Category: Molecular Biology Source Type: research

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Publication date: Available online 21 June 2019Source: Food ControlAuthor(s): Emalie Thomas-Popo, Aubrey Mendonca, James Dickson, Angela Shaw, Shannon Coleman, Aura Daraba, Armitra Jackson-Davis, Floyd WoodsAbstractThe antimicrobial effectiveness of isoeugenol against Escherichia coli O157:H7, Salmonella enterica, and Listeria monocytogenes in refrigerated (4 °C) pineapple juice (PJ) with added 0.5% (w/v) yucca extract (YEX) was investigated. Sub-lethal injury of pathogen survivors and sensory characteristics of PJ were also evaluated. Samples of PJ with YEX and isoeugenol at 0 (control), 0.50, 0.75, 1.0, or 1.5 &m...
Source: Food Control - Category: Food Science Source Type: research
Publication date: Available online 21 June 2019Source: Journal of BiotechnologyAuthor(s): Chen Zhang, Ying Zang, Peng Liu, Zhaojuan Zheng, Jia OuyangAbstract4-Coumarate: CoA ligase (4CL) is an important branch point directing metabolites to flavonoid or monolignol pathways in plants. It plays a vital role in the biosynthesis of plant nature products in microbes. Herein, Ptr4CL4, Ptr4CL5 and Ptr4CL7 from Populus trichocarpa were cloned and expressed in Escherichia coli. Two recombinant proteins Ptr4CL4 and Ptr4CL5 showed distinct activities for different substrates. The Ptr4CL4, not previously reported, showed the hi...
Source: Journal of Biotechnology - Category: Biotechnology Source Type: research
Publication date: Available online 17 June 2019Source: Journal of Hazardous MaterialsAuthor(s): Otto Lucas Heinz, Mário A.A. Cunha, Jéssica S. Amorim, Aneli M. Barbosa-Dekker, Robert F.H. Dekker, Marcio Barreto-RodriguesAbstractThe present study reports on the remediation of an effluent from the wood-laminate industry using Pleurotus ostreatus EB 016 in combination with photo-Fenton oxidation. Fermentation of the effluent with P. ostreatus EB-016 was carried out in agitated flasks to evaluate the influence of pH, and concentrations of carbon and nitrogen sources by a multivariate approach. Subsequently, bioas...
Source: Journal of Hazardous Materials - Category: Environmental Health Source Type: research
We report an observation of a febrile gastroenteritis complicated by signs of intra and extracellular dehydration, in a 37-year-old lupus patient, including duodenal biopsies, and stool parasitology, which led to the diagnosis of strongyloidiasis effectively treated by ivermectin per os (two doses) of 200 micrograms/kg, once every 2 weeks apart), following failure of a first 5-days course of albendazole (400 mg/day). PMID: 31225731 [PubMed - in process]
Source: Bulletin de la Societe de Pathologie Exotique - Category: Tropical Medicine Tags: Bull Soc Pathol Exot Source Type: research
In this study, we investigated the bacterial biofilm reduction by combined treatment of atmospheric pressure plasma (APP) and chelating agents. Many of hospital acquired infections (HAI) are related to biofilm infections. APP and chelating agents were reported to be effective in bacterial biofilm inactivation and eradication. It is believed that chelating agents disrupt the biofilm formation through bonding metal ions, while APP inactivates bacteria mainly through reactive species [1], [2], [3]. In our study, we used a surface micro-discharge (SMD) driven by sinusoidal power input of 2 kHz and peak to peak voltage of 9 kV ...
Source: Clinical Plasma Medicine - Category: Research Source Type: research
Authors: Ishaq R, Ansari MS, Rakha BA, Qadeer S, Akhter S Abstract Aim: The present study was designed to evaluate enrofloxacin (EN), a fluoroquinolone broad-spectrum antibacterial drug for use in cryopreservation of Zebu bull semen. Materials and Methods: Semen was collected from three Zebu bulls of the Sahiwal breed (Bos indicus) for 3 weeks (replicates) for each experiment. Experiment I: semen samples were cultured for isolation of bacteria and in vitro sensitivity tests against streptomycin, penicillin, and EN. Proteus spp., Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli were isolated. P. a...
Source: Biopreservation and Biobanking - Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research
Authors: Picking WD, Barta ML Abstract Type III secretion systems are used by some Gram-negative bacteria to inject effector proteins into targeted eukaryotic cells for the benefit of the bacterium. The type III secretion injectisome is a complex nanomachine comprised of four main substructures including a cytoplasmic sorting platform, an envelope-spanning basal body, an extracellular needle and an exposed needle tip complex. Upon contact with a host cell, secretion is induced, resulting in the formation of a translocon pore in the host membrane. Translocon formation completes the conduit needed for effector secret...
Source: Current Topics in Microbiology and Immunology - Category: Microbiology Tags: Curr Top Microbiol Immunol Source Type: research
Authors: Sethuvel DPM, Anandan S, Michael JS, Murugan D, Neeravi A, Verghese VP, Walia K, Veeraraghavan B Abstract Shigella is the major cause of bacillary dysentery worldwide, especially in developing countries. There are several virulence factors essential for the organism to be virulent which are generally present in the virulence plasmid and on chromosomal pathogenicity islands. The present study was undertaken to determine the virulence gene profile of Shigella spp isolated from a clinical specimen and to study their significant association with common clinical symptoms and antimicrobial resistance. Sixty Shig...
Source: Pathogens and Global Health - Category: International Medicine & Public Health Tags: Pathog Glob Health Source Type: research
Publication date: November 2019Source: Carbon, Volume 152Author(s): Pranjali Yadav, S.T. Nishanthi, Bhagyesh Purohit, Asifkhan Shanavas, Kamalakannan KailasamAbstractGraphitic carbon nitride (g-CN, commonly called as g-C3N4) quantum dots exhibiting excellent photoluminescence behaviour is explored as metal-free visible light photocatalytic antibacterial agents. The g-CNQDs were synthesized by two-step thermal polymerization and selective dialysis approach yielding particle size in the range of 2–7 nm with a large specific surface area of 264 m2/g. The fluorescent g-CNQDs could efficiently generate superoxide and ...
Source: Carbon - Category: Materials Science Source Type: research
Folding of β-Barrel Membrane Proteins into Lipid Membranes by Site-Directed Fluorescence Spectroscopy. Methods Mol Biol. 2019;2003:465-492 Authors: Gerlach L, Gholami O, Schürmann N, Kleinschmidt JH Abstract Protein-lipid interactions are important for folding and membrane insertion of integral membrane proteins that are composed either of α-helical or of β-barrel structure in their transmembrane domains. While α-helical transmembrane proteins fold co-translationally while they are synthesized by a ribosome, β-barrel transmembrane proteins (β-TMPs) fold and insert ...
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research
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