The cell death phenotype of MGC-803 cells inducing with"dextran-magnetic layered double hydroxide-fluorouracil" drug delivery system and fluorouracil.

The cell death phenotype of MGC-803 cells inducing with"dextran-magnetic layered double hydroxide-fluorouracil" drug delivery system and fluorouracil. Biol Pharm Bull. 2019 May 18;: Authors: Pei Q, Wang R, Shu C, Pei X, Li X, Gou G Abstract The purpose of the paper is to study the differences in cell death mechanism of MGC-803 induced by "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) drug delivery system and 5-Fluorouracil (5-Fu), respectively. The inhibitory effect on the proliferation was detected via CCK-8. The morphology of cell death was detected by transmission electron microscopy (TEM). Intracellular ATP, mitochondrial membrane potential (MMP), reactive oxygen species (ROS) and Cytosolic Free Ca (Ca2+) level were detected via some methods. The result showed that DMF had more obvious effect in suppressing proliferation compared with 5-Fu, and changed cell death pattern of 5-Fu from apoptosis to oncosis. The ATP decrease, MMP loss, Ca2+ increase, the activation of UCP-2 and caplain-1 were significant after DMF exposure. However, DMF did not result in ROS accumulation. DMF could involve in activation of porimin, and the cascade reaction of caspase-3, 7, 9, 12 and PARP through western blot. DMF showed a stronger injury on nuclear membrane in the cascade reaction of caspase-6, caspase-8 and lamin-A. DMF triggered rapid depletion of ATP, which was consistent with the phenotype of oncosis. Endogenous mitochondrial ap...
Source: Biological and Pharmaceutical Bulletin - Category: Drugs & Pharmacology Authors: Tags: Biol Pharm Bull Source Type: research