Protective effects on age-related macular degeneration by activated autophagy induced by amyloid- β in retinal pigment epithelial cells.

This study was performed to detect the activation of autophagy in retinal pigment epithelial (RPE) cells treated with Aβ, aiming to investigate the potential protective mechanism of RPE cells against Aβ stress. Human ARPE-19 cells were treated with soluble Aβ1-42 oligomer. Transmission electron microscopy was used to assess the formation of autophagic compartments; immunofluorescence was used to detect the subcellular localization of LC3; and western blot was used to detect the conversion of LC3-I to LC3-II and the expression of p62. Activated autophagy in Aβ-treated ARPE-19 cells was detected by three methodologies: 1) generation of autophagic compartments by transmission electron microscopy, 2) altered expression pattern of LC3 by immunofluorescence, and 3) elevated light-chain-3 II (LC3-II) and decreased p62 expression by western blot. These results suggest that Aβ could induce autophagy in RPE cells, which provided a potential protective mechanism for the retina cells that encountered Aβ deposition. PMID: 31095924 [PubMed - in process]
Source: Discovery Medicine - Category: Research Tags: Discov Med Source Type: research