Label Free Proteomic Analysis Reveals Large Dynamic Changes to the Cellular Proteome Upon Expression of the miRNA-23a-27a-24-2 MicroRNA Cluster.

Label Free Proteomic Analysis Reveals Large Dynamic Changes to the Cellular Proteome Upon Expression of the miRNA-23a-27a-24-2 MicroRNA Cluster. Biochem Cell Biol. 2019 May 16;: Authors: Piragasam RS, Hussain SF, Chaulk SG, Siddiqi ZA, Fahlman RP Abstract In deciphering the regulatory networks of gene expression controlled by the small non-coding RNAs known as microRNAs (miRNAs), a major challenge has been with the identification of the true mRNA targets by these RNAs within the context of the enormous numbers of predicted targets for each of these small RNAs. To facilitate the system wide identification of miRNA targets a variety of system wide methods, such as proteomics, have been implemented. Here we describe the utilization of quantitative label-free proteomics and bioinformatics to identify the most significant changes to the proteome upon expression of the miR-23a-27a-24-2 miRNA cluster. In light of recent work leading to the hypothesis that only the most pronounced regulatory events by miRNAs may be physiologically relevant, our data reveals that label-free analysis circumvents the limitations of proteomic labeling techniques that limit the maximum differences that can be quantified. The result of our analysis identifies a series of novel candidate targets that are reduced in abundance by more than an order of magnitude upon the expression of the miR-23a-27a-24-2 cluster. PMID: 31095918 [PubMed - as supplied by publis...
Source: Biochemistry and Cell Biology - Category: Biochemistry Authors: Tags: Biochem Cell Biol Source Type: research