A molecular investigation into the interaction of SiO2 nanoparticles with elastase by multispectroscopic techniques and kinetic studies.

A molecular investigation into the interaction of SiO2 nanoparticles with elastase by multispectroscopic techniques and kinetic studies. Int J Biol Macromol. 2019 May 03;: Authors: Sadeghi-Kaji S, Shareghi B, Saboury AA, Farhadian S, Hemmati R Abstract The increasing use of nanoparticles in various industries has triggered the need to study their interconnection with biological macromolecules. The goal of this study was to survey the SiO2-nanoparticles efficacy on the thermal denaturation, conformation and activity of elastase at three temperatures of 303, 313 and 323 K in the Tris buffer at pH of 8.5. In this work, distinct techniques such as UV-vis Spectrophotometry, Spectrofluorometry, and circular dichroism were employed. The fluorescence studies showed that SiO2 nanoparticles extremely reduced the intensity of elastase with the static mechanism. Thermodynamic parameter analysis also indicated that the process of binding of SiO2-nanoparticles to elastase was spontaneous, thereby suggesting that van der Waals forces or hydrogen bonding interactions played a key role in determining the complex stability. Far-UV circular dichroism studies further revealed that SiO2 nanoparticles could cause 9.79% reduction in the content of the α-helix and 3.24% increase in the content of the β-sheet. Furthermore, kinetic parameters (Vmax and Kcat/Km) indicated that SiO2 nanoparticles had an activation effect on the elastase activity. Melting te...
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research