Norovirus Detection at Oualidia Lagoon, a Moroccan Shellfish Harvesting Area, by Reverse Transcription PCR Analysis

AbstractNorovirus (NoV) is the leading cause of acute viral gastroenteritis outbreaks in the world. These outbreaks are frequently associated with bivalve shellfish consumption, particularly because these products are often eaten raw or only slightly cooked. In Morocco, regulations concerning the acceptable levels of enteric bacteria indicator organisms in these products have been put in place. However, these regulations do not take into account the risk of viral contamination, and many gastroenteritis outbreaks have been linked to the ingestion of bivalve shellfish from areas that comply with the current food safety criteria. The aim of this study was to investigate NoV presence in shellfish samples (n = 104) collected at four sites owcff Oualidia lagoon (Moroccan Atlantic coast) from November 2015 to February 2017. Samples were analysed using real-time RT-PCR in accordance with the ISO 15216-2 method. NoVs of the genogroup II were detected in 7% of samples that were all collected during the winter months. Moreover, 71% of NoV-positive samples were harvested at sites upstream of the lagoon. These results highlight the need of regularly monitoring viral contamination in bivalve shellfish to limit the risk of viral gastroenteritis outbreaks.
Source: Food and Environmental Virology - Category: Virology Source Type: research

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ConclusionsVarious HuNoV genotypes including GII.2, GII.4, GII.6, and GII.17 were shown to be associated with various types of outbreak sites (at childcare and educational facilities, involving cases of food poisoning, and at elderly nursing homes) in this study. These genotypes emerged in recent years, and their prevalence patterns differed from each other. Moreover, differences in outbreak sites and viral load of patients among the genotypes were identified.
Source: Gut Pathogens - Category: Microbiology Source Type: research
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Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research
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Source: Euro Surveill - Category: Infectious Diseases Authors: Tags: Euro Surveill Source Type: research
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Source: The Journal of Infection in Developing Countries - Category: Infectious Diseases Source Type: research
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Source: Food Microbiology - Category: Food Science Authors: Tags: Food Microbiol Source Type: research
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Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research
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Source: Viruses - Category: Virology Authors: Tags: Review Source Type: research
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Source: Praxis - Category: General Medicine Authors: Tags: Praxis (Bern 1994) Source Type: research
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Source: Food and Environmental Virology - Category: Virology Source Type: research
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Source: Journal of Virological Methods - Category: Virology Authors: Tags: J Virol Methods Source Type: research
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