Multi-walled carbon nanotubes reinforced into hollow fiber by chitosan sol-gel for solid/ liquid phase microextraction of NSAIDs from urine prior to HPLC-DAD analysis.

Multi-walled carbon nanotubes reinforced into hollow fiber by chitosan sol-gel for solid/ liquid phase microextraction of NSAIDs from urine prior to HPLC-DAD analysis. Curr Pharm Biotechnol. 2019 Apr 05;: Authors: Al-Hashimi NN, El-Sheikh AH, Qawariq RF, Shtaiwi MH, AlEjielat R Abstract The efficient analytical method for analysis of nonsteroidal anti-inflammatory drugs (NSAIDs) in biological fluid are important for determining the toxicological aspects of such long-term used therapies. In the present work, multi-walled carbon nanotubes reinforced into hollow fiber by chitosan sol-gel assisted-solid/ liquid phase microextraction (MWCNTs-HF-CA-SLPME) method followed by high performance liquid chromatography-diode array detection (HPLC-DAD) was developed for determination of three NSAIDs, ketoprofen, diclofenac, and ibuprofen in human urine samples. MWCNTs with various dimensions were characterized by various analytical techniques. The extraction device was prepared by immobilizing the MWCNTs in the pores of 2.5 cm microtube via chitosan sol-gel assisted technology while the lumen of the microtube was filled with few microliters of 1-octanol with two ends sealed. The extraction device was operated by direct immersion in the sample solution. The main factors influencing the extraction efficiency of the selected NSAIDs have been examined. The method showed good linearity R2 ≥ 0.997 with RSDs from 1.1 to 12.3%. The limit of detection (L...
Source: Current Pharmaceutical Biotechnology - Category: Biotechnology Authors: Tags: Curr Pharm Biotechnol Source Type: research