Long noncoding RNA MALAT1 regulates sepsis in patients with burns by modulating miR ‑214 with TLR5.

Long noncoding RNA MALAT1 regulates sepsis in patients with burns by modulating miR‑214 with TLR5. Mol Med Rep. 2019 Mar 14;: Authors: Gao F, Chen R, Xi Y, Zhao Q, Gao H Abstract The present study aimed to identify the involvement of the dysregulation of the metastasis‑associated lung adenocarcinoma transcript‑1 (MALAT1)/microRNA (miR)‑214/Toll‑like receptor (TLR)5 signaling pathway in the development of post‑burn sepsis. THP‑1 cells were used in the present study, in addition to 8‑10 week‑old mice. ELISA analysis was performed to examine the expression levels of inflammation‑associated factors. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis and western blotting were performed to analyze the influence of burns or burns with infection on the production of MALAT1, miR‑214 and TLR5. Commonly‑used software and a luciferase assay was used to confirm the target gene of miR‑214. RT‑qPCR analysis and western blotting were performed to elucidate the effects of lipopolysaccharide (LPS), miR‑214 and MALAT1 on the expression of miR‑214, TLR5, tumor necrosis factor (TNF)‑α, interleukin (IL)‑6 and IL‑10. Burn injury increased TLR5, TNF‑α, IL‑6 and IL‑10 expression levels, which were abolished by treatment with MALAT1. miR‑214 directly targeted TLR5 by binding to the TLR5 3' untranslated region (ITR), and the luciferase activity of the wild‑type, and not the mut...
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research