Voltammetric determination of 17β-estradiol in different matrices using a screen-printed sensor modified with CuPc, Printex 6L carbon and Nafion film

Publication date: June 2019Source: Microchemical Journal, Volume 147Author(s): Ademar Wong, Anderson Martin Santos, Elson Luiz Fava, Orlando Fatibello-Filho, Maria Del Pilar Taboada SotomayorAbstractA screen-printed disposable electrode made of carbon ink modified with CuPc, P6LC and Nafion film is proposed as a low-cost and simple analytical method for sensitive determination of 17β-estradiol in different matrices. The electrochemical behaviour of 17β-estradiol was evaluated by cyclic voltammetry in the potential range from 0 to 0.55 V at a scan rate of 0.05 V s−1 using 0.1 mol L−1 phosphate buffer (pH 5.0) as supporting electrolyte. One oxidation peak was observed during anodic potential scanning, at a potential of 0.39 V. Under optimized experimental conditions, using the differential pulse voltammetry (DPV) technique, the proposed sensor showed a linear concentration range for 17β-estradiol between 8.0 × 10−8 to 7.3 × 10−6 mol L−1, and detection limit of 5.0 × 10−9 mol L−1.The CuPc-P6LC-Nafion/SPEF sensor showed good selectivity, sensitivity, stability, reproducibility, and repeatability; it is a low-cost disposable device and shows no interference in DPV experiments. The proposed sensor was successful when applied to environmental and synthetic urine samples, with recoveries close to 100%.
Source: Microchemical Journal - Category: Chemistry Source Type: research