Phospholipase C signaling is involved in porcine reproductive and respiratory syndrome virus infection in cell cultures.

In this study, we demonstrated that PLC-specific inhibitor U73122 strongly suppressed porcine reproductive and respiratory syndrome virus (PRRSV) productive infection in cell cultures. The inhibitor affected both viral post-binding cell entry and post-entry processes. The virus infection led to an early transient activation of PLCγ-1 at 0.5 h post-infection (hpi), and sustained event at a stage from 4 to 16 hpi in MARC-145 cells. In addition, U73122 inhibited the activation of p38 MAPK signaling stimulated by PRRSV infection, suggesting that PLC signaling may be associated with the virus infection-induced inflammatory response. Taken together, these studies suggested that PLC signaling played an important role in PRRSV infection or pathogenesis. Keywords: PRRSV; U73122; phospholipase C; PLCγ-1.
PMID: 30879321 [PubMed - in process]

Source: Acta Virologica - March 19, 2019 Category: Infectious Diseases Authors: Yuan WF, Chen Q, Gao XT, Zheng ZM, Jia H, Zhu HF, Xin T, Sui XK, Li M, Hou SH, Guo XY Tags: Acta Virol Source Type: research